摘要
目的研究家族成员中反复腹痛和无反复腹痛患者的幽门螺杆菌(Hp)感染和人类白细胞抗原(HLA)-DQA1等位基因频率分布。方法应用胶体金标免疫渗滤法和免疫印迹法检测20个反复腹痛患儿家族118名成员血Hp—IgG抗体和亚型。118人被分为Hp感染有腹痛症状组69例和无腹痛症状组49例;用聚合酶链反应一序列特异性引物(PCR—SSP)方法对两组家族成员进行HLA—DQB1和DRB1基因分型检测。结果20个家族中Hp抗体和Hp亚型阳性率分别为100%和96.6%,Ⅰ型Hp感染占55.1%,Ⅱ型Hp感染为41.5%。家族中Hp感染有腹痛症状组HLA-DQB1*09和DRB1%09的等位基因频率高于无腹痛症状组,但经等位基因多项比较校正后差异消失(14%vs6%,x^2=4.012,P=0.045,Pc〉0.05;10%VS5%,x^2=5.636,P=0.018,Pc〉0.05)。结论在Hp感染的家族成员中,反复腹痛者与无腹痛者之间存在免疫遗传学差异,HLA—DQB1*09和DRB1*09等位基因和Hp感染后反复腹痛的相关性需深入研究。
Objective To investigate Helicobacter pflori (Hp) infection and human leukocyte antigen (HLA) DQB1 and DRB1 allelic frequencies among l l8 members with and without recurrent abdominal pain in the families. Methods The serum Hp antibody and immunophenotyping was tested and determined by dot im- munogold filtration assay and Western blot technique among 118 familial members of 20 familles with recurrent abdominal pain. 118 members were divided into two groups: 69 members with recurrent abdominal pain. and 49 without such a symptom. PCR-SSP technique was applied to identify HLA-DQB1 and DRB1 allelic frequencies between two groups. Result The Hp sero-positive rate in 118 members was 100% and the Hp immunophenotyping was 96.6%. The prevalence of I and II types of Hp were 55.1% and 41.5%. HLA-DQB1 * 09 and DRB1 * 09 allele frequencies were higher in the members with recurrent abdominal pain than that in the members without recurrent abdominal pain, but there was no significant difference after the allelic Bonferroni correction for comparisons were applied (14% vs 6%, X2 = 4. 012,P = 0. 045 ,Pc 〉 0.05;10% vs 5%, X2 = 5. 636, P = 0. 018,Pc 〉 0.05, respectively). Conclusion There is immunogenetic difference between faimial members with and without recurrent abdominal pain infected by Hp. The association between HLA-DQB1 * 09 and DRB1 * 09 allele and the recurrent abdominal pain needs further study.
出处
《国际免疫学杂志》
CAS
2012年第1期71-76,共6页
International Journal of Immunology
基金
云南省中青年学术技术带头人后备人才培养计划资助项目(2006PY1-18):云南省自然科学研究应用基金项目(2009CD163)