摘要
目的观察脑通胶囊对血管性痴呆(VD)模型大鼠学习记忆、海马组织N-甲基-D-天冬氨酸(NMDA)受体1亚基和2B亚基mRNA表达的影响。方法采用改良的四血管法(14-VO)制备VD模型,Morris水迷宫测定大鼠学习、记忆能力,实时荧光定量PCR检测NMDA受体1亚基和2B亚基mRNA的表达情况。结果与模型组比较,脑通胶囊大、中剂量组逃避潜伏期缩短,穿越原平台次数增加,NMDA受体1亚基mRNA表达降低,NMDA受体2B亚基mRNA表达升高(P<0.05,P<0.01)。结论脑通胶囊可降低NMDA受体1亚基mRNA的表达,提高NMDA受体2B亚基mRNA的表达,从而改善VD大鼠学习、记忆能力。
Objective To observe the effect of Naotong capsule on the learning and memory abilities, the mRNA expression level of subunit 1 and 2B of NMDA receptor in the hippocampus of vascular dementia （VD） model rats. Methods VD models were established by modified Pulsinelli 4 vascular occlusions method （4VO）. Morris water maze was used to test learning and memory abilities of rats. And the mRNA expression levels of subunit 1 and 2B of NMDA receptor were determined by the real-time quantitative PCR. Results Compare to the model group, the escape time shortened, times of spanning the previous platform increased, the mRNA expression level of subunit 1 decreased and the mRNA expression level of subunit 2B increased in Naotong capsule large and middle-dose treatment groups （ P 〈 0. 05, P 〈 0. 01 ）. Conclusions Naotong capsule can improve learning and memory abilities of VD rats by decreasing the mRNA expression level of subunit 1 and increasing the mRNA expression level of subunit 2B of NMDA receptor.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2012年第1期79-82,共4页
Chinese Journal of Gerontology
基金
贵州省科技攻关计划资助项目(黔科合SY字〔2008〕3048号)
贵州省中药产业现代化专项资金资助项目(黔科合农字〔2006〕5023号)
教育部春晖计划项目(Z2005-2-5027)
贵州省中医药管理局资助项目(黔中医药2007020)
关键词
脑通胶囊
血管性痴呆
四血管法
NMDA受体
Naotong capsule
Vascular dementia
4 vascular occlusions
NMDA receptor
