摘要
目的探讨不同浓度异氟醚后处理对新生大鼠缺血缺氧性脑损伤的影响。方法 7 d龄新生SD大鼠125只随机分为5组(n=25):假手术组(Ⅰ组)、脑缺血缺氧组(Ⅱ组)、1%异氟醚后处理组(Ⅲ组)、1.5%异氟醚后处理组(Ⅳ组)和2%异氟醚后处理组(Ⅴ组)。除Ⅰ组外,其余各组均行左颈总动脉结扎和8%低氧2 h处理,制成新生大鼠缺血缺氧性脑损伤模型。Ⅲ、Ⅳ和Ⅴ组脑损伤后即刻分别吸入1%、1.5%和2%异氟醚30 min。于脑损伤后7 d测各组新生鼠的左右大脑半球质量,并行脑组织损伤病理检测。结果与Ⅰ组相比,其余4组的左/右大脑半球质量比及丘脑腹后内侧核和后扣带回皮层左/右侧神经元密度的比值降低(P<0.05);与Ⅱ组相比,Ⅳ和Ⅴ组左/右大脑半球质量比及丘脑腹后内侧核和后扣带回皮层左/右侧神经元密度比降低的程度减轻(P<0.05)。结论 1.5%和2%异氟醚后处理能够减轻新生大鼠缺血缺氧性脑损伤程度。
Objective To investigate the effects of different doses of isoflurane postconditioning on neonatal hypoxic/ischemic brain injury in rats. Methods 125 seven-day-old SD rats were randomly divided into 5 groups （n =25）：group I ：sham surgery,the pups of other four groups had left common carotid arterial ligation followed by hypoxia with 8% oxygen for 2 h at 37 ℃. Isoflurane posteonditioning with 1% （group III ）,1.5% （group IV ） or 2% （group V ） isoflurane for 30 rain was performed immediately after the brain hypoxia/isehemia. The weight ratio, neuronal density ratio in the ventral posteromedial thalamic nucleus and the retrosplenial granular cortex of left to right cerebral hemispheres at 7 days after the brain hypoxia/ischemia was calculated. Results Compared with control （group I ）,the weight ratio and neuronal density ratio in the ventral posteromedial thalamic nucleus and in the retrosplenial granular cortex of left/right cerebral hemispheres in other four groups decreased （P 〈 0.05）. Compared with group II ,the decreasing level in the weight ratio and neuronal density ratio in the ventral posteromedial thalamic nucleus and in the retrosplenial granular cortex of left/right cerebral hemispheres induced by hypoxia/ischemia in group IV and V reduced （P〈 0.05）. Conclusion 1.5% and 2% isoflnrane postconditioning induced neuroprotection in neonatal rats of brain hypoxia and ischemia injury.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2012年第1期5-7,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(81171782)
关键词
异氟醚
后处理
缺血缺氧性脑损伤
新生大鼠
isoflurane
postconditioning
hypoxic/ischemic brain injury
neonatal rat
