摘要
目的 探讨EBV立刻早期蛋白Zta基因对Daudi细胞周期的影响及其可能机制.方法 构建Zta基因真核表达载体,通过电穿孔将该载体转染Daudi细胞,用流式细胞术检测细胞周期的变化,用蛋白印迹试验检测细胞周期蛋白p21、Rb、E2F-1的表达.结果 成功构建了 Zta基因表达载体,转染Zta基因可抑制Daudi细胞的增殖,并促进Daudi细胞由G0/G11期[（30.0±3.4）％）]向S期[（47.7±1.1）％]的转化.同时转染Zta基因可下调Rb的表达、上调E2F-1、p21的表达.结论 转染Zta基因使Daudi细胞周期由Go/G1期向S期转变,其机制可能与Rb的表达下降。
Objective To investigate the effect of EBV imrnediate-early protein Zta on cell cycle ofDaudi cells and the involved mechanisms. Methods The expression vector encoding Zta was constructed and electroporated into Daudi ceils. Flow cytometric analysis was used to detect the cell cycle, Western blot to the protein levels of p21, Rb and E2F-1. Results The vector was constructed successfully, the expression of Zta protein inhibited the proliferation of Daudi cells and promoted cell cycle from Gο/G1 phase [ （30. 0 ± 3.4） % ] to S phase [ （47.7 ± 1.1 ）% ]. Meanwhile, Rb expression was significantly downregulated, E2F-1 and IO21 expression upregulated by Zta. Conclusion Zta could promote Gο/G1 phase to S phase transition in Daudi cells,which might be associated with the reduced expression of Rb and increased expression of E2F-1 and p21 protein.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
2012年第1期47-50,共4页
Chinese Journal of Hematology
