摘要
目的 探讨支气管哮喘大鼠肺内神经营养因子水平的变化,及其与气道神经可塑性改变和气道高反应性间的关系. 方法 雄性SD大鼠40只按随机数字表法分为对照组、哮喘组、神经生长因子（NGF）＋脑源性神经营养因子（BDNF）干预组和anti-NGF＋anti-BDNF干预组（每组各10只）,后三组通过鸡卵蛋白致敏激发建立哮喘模型并给予相应的干预.造模后第8周进行气道阻力测定,并留取左肺行HE染色及免疫组化染色检测肺组织中神经营养因子如NGF、BDNF的表达水平,RT-PCR法检测气道突触囊泡素（SYN）mRNA和神经丝（NF）mRNA的表达水平. 结果 哮喘组及NGF＋BDNF干预组大鼠肺组织内可见大量炎症细胞浸润.哮喘组大鼠肺组织中NGF及BDNF的表达水平均明显高于对照组及anti-NGF＋anti-BDNF干预组,差异有统计学意义（P＜0.05）,而NGF＋BDNF干预组肺组织中NGF及BDNF的表达水平明显高于哮喘组,差异亦有统计学意义 （P＜0.05）.哮喘组及NGF＋BDNF干预组较对照组的气道反应性增高,气道感觉神经元SYN mRNA及NF mRNA的表达水平也明显增高,差异均有统计学意义（P＜0.05）.哮喘组大鼠肺组织中NGF及BDNF浓度与气道SYN表达呈正相关关系（r=0.889,P＜0.05；r=0.985,P＜0.05）,与NF表达呈正相关关系（r=0.956,P＜0.05；r=0.927,P＜0.05）,与气道反应性也呈正相关关系（r=0.938,P＜0.05；r=0.906,P＜0.05）. 结论 NGF及BDNF可能参与了支气管哮喘大鼠气道神经可塑性改变的调节过程,从而导致气道高反应性的发生.
Objective To investigate the effect of change in neurotrophins level in the bronchial asthma rats＇ lung on airway hyperresponsiveness and airway neural plasticity. Methods A total of forty male SD rats were randomly divided into 4 groups with 10 rats in each group： control group,asthmatic group, NGF＋BDNF prevention group and anti-NGF＋anti-BDNF prevention group. The asthmatic model was established by inhalation and injection of ovalbumin. The airway responsiveness was measured after 8 weeks.The bronchial inflammation was assessed by HE staining,and nerve growth factor and brain derived neurotrophic factor expressions of left lung were assayed by the immunohistochemistry staining.Then the expressions of synaptophysin and neurofilament were detected by RT-PCR. Results Compared with the control group, the lung tissue of the asthma group and NGF＋BDNF prevention group had more infiltrating inflammatory cells; The expressions of NGF and BDNF were higher in the asthma group and NGF＋BDNF prevention group than those in the control group and anti-NGF＋anti-BDNF prevention group （P〈0.05）,and significantly higher in the NGF＋BDNF prevention group than those in the asthma group （P〈0.05）.Both the airway responsiveness and the levels of SYN mRNA and NF mRNA in the lung tissues were significantly higher in the asthma group and NGF＋BDNF prevention group than those in the control group （P〈0.05）.In asthma group,the expressions of NGF and BDNF were positively related to the expressions of SYN （r=0.889,P〈0.05; r=0.985,P〈0.05）and NF（r=0.956,P〈0.05; r=0.927,P〈0.05）,and also positively related to the airway hyperresponsiveness （r=0.938,P〈0.05; r=0.906,P〈0.05）. Conclusion NGF and BDNF might be involved in rat airway bronchial neural plasticity changes,which resulted in the airway hyperresponsiveness.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2012年第1期31-36,共6页
Chinese Journal of Neuromedicine
基金
国家自然科学基金（30960143）
江西省卫生厅科技计划资助项目（20081005）
