摘要
This study aims to investigate the effects of a probe and a cup horn on the de-agglomeration efficiency in ultrasound vibration processes. TiO2 and Al2O3 nanoparticle dispersions were prepared in distilled water at a concentration of 50.0 mg/mL followed by treatment with a dispersion stabilizer （100% FBS） and ultrasound vibration at 20 kHz and 35% amplitude for 10 min by a probe and a cup horn, respectively. The average sizes of dispersed TiO2 and Al2O3 nanoparticles were measured by a dynamic light scattering device. Compared to dispersion with the probe sonicating, the average sizes of TiO2 and Al2O3 particles sonicated by the cup horn are markedly smaller at time points of 30, 60, 120, and 180 min. The TiOe and Al2O3 particle size distributions of cup horn-treated suspensions were narrower than those of probe-treated suspensions at time points of 120 and 180 min. It is suggested that the cup horn has a higher efficiency than the probe in dispersing nanoparticles, The cup horn is better than the probe for processing multiple small sample vessels simultaneously. Indirect cup horn sonication is ideal for processing pathogenic and sterile samples.
比较了探针式和杯式超声探头对纳米颗粒的超声分散效果.用蒸馏水将纳米二氧化钛和纳米三氧化二铝配制成浓度为50.0mg/mL的混悬液,随后加入分散稳定剂(100%胎牛血清),分散液涡旋振荡2min后备用.分别采用探针式和杯式探头以20kHz,35%振幅超声波将上述2种混悬液振荡10min,用动态光散射法分析二氧化钛和三氧化二铝纳米颗粒的粒径大小及其分布情况.结果表明,杯式探头超声波振荡组在30,60,120,180min时间点的二氧化钛和三氧化二铝平均粒径均明显小于探针式探头超声波振荡组.同时,杯式探头超声波振荡组在120和180min时间点的二氧化钛和三氧化二铝颗粒粒径分布范围均窄于探针式探头超声波振荡组.结果说明杯式超声探头比探针式超声探头对纳米颗粒具有更好的分散效果.杯式探头在同一时间能处理多个小容积样本且特别适合于制备有致病危险性的样品或无菌样品.
基金
The National Basic Research Program of China(No.2011CB933404)
the Foundation of Jiangsu Key Laboratory for Biomaterials and Devices(No.2010LBMD05)
