摘要
利用离子交换层析和凝胶层析对浒苔多糖进行分离纯化,并探讨其磷酸化修饰的最佳工艺条件。通过单因素试验确定反应温度、反应时间、反应pH值、磷酸化试剂质量浓度的取值范围,再用响应面设计法确定磷酸化修饰的最佳条件。结果表明:反应时间、磷酸化试剂质量浓度显著影响浒苔多糖磷酸化程度,当反应温度80℃、反应时间5h、反应pH9.0、磷酸化试剂质量浓度0.10g/mL时,磷酸化修饰的浒苔多糖磷酸根含量(以P计)达到14.40%。
This study was undertaken to optimize the phosphorylation of Enteromorph polysaccharides prepared by hot water extraction,deproteinization,decolorization,and sequential chromatographic purification on DEA-Sepharose Fast Flow and Sephadex G-100 columns.Phosphate group content in phosphorylated products was investigated with respect to reaction conditions including temperature,reaction time,pH and phosphorylation agent dosage（sodium tripolyphosphate-sodium trimetaphosphate at a mass ratio of 6：1） by one-factor-at-a-time experiments and subsequent response surface analysis.The optimal phosphorylation conditions were pH 9.0,temperature 80 ℃ and phosphorylation agent dosage 0.10 g/mL for a reaction duration of 5 h.Under these conditions,the phosphate group content in phosphorylated products was 14.40%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第24期73-77,共5页
Food Science
基金
浙江省自然科学基金重点项目(Z3110211)
关键词
浒苔多糖
磷酸化
响应面法优化
polysaccharides from Entermorpha
phosphorylation
response surface methodology（RSM）
