摘要
背景:前交叉韧带是膝关节常见损伤部位,组织工程前交叉韧带研究在过去的10年内取得了长足的进展,但人们尚未找到理想的支架材料。目的:探讨使用可降解聚氨酯材料构建组织工程交叉韧带支架的可行性。方法:将可降解聚氨酯纤维丝编织的聚氨酯材料于含胎牛血清的DMEM培养基制备浸提液,分别以体积分数100%,50%,25%,12.5%的浸提液培养NIH3T3细胞,CCK-8实验检测聚氨酯材料浸提液的细胞毒性;然后将体外分离培养的兔前交叉韧带细胞和NIH3T3细胞分别种植在支架材料上进行立体培养,观察细胞在支架材料上的生长情况;使用电子拉力机测试支架材料的力学性能并分析。结果与结论:构建组织工程交叉韧带支架的可降解聚氨酯材料没有细胞毒性;前交叉韧带和NIH3T3细胞贴附于该材料生长,生长状态良好;支架材料的杨氏模量,极限抗张强度和断裂伸长率分别为(41.2±2.1)MPa,(51.0±1.5)MPa和(600±60)%。提示聚氨酯支架材料具有良好的生物相容性和生物安全性。
BACKGROUND： The anterior cruciate ligament （ACL） of the knee joint is the common site of injury. Tissue engineering has achieved much progress in the past 10 years for ACL, but none of the ideal scaffolds. OBJECTIVE： To investigate the feasibility of biodegradable polyurethane in construction of tissue engineering ACL. METHODS： Leaching liquor of polyurethane composed of biodegradable polyurethane fibers was prepared on DMEM medium containing fetal bovine serum. NIH3T3 cells were cultured in DMEM medium with 100%, 50%, 25%, and 12.5% of leaching liquor. The cytotoxicity of polyurethane was detected by CCK-8 agent. Rabbit ACL cells and NIH3T3 cells were isolated and cultured in vitro, and seeded onto the surface of the polyurethane scaffold to observe their growth. The biomechanical properties of the polyurethane scaffold were measured and analyzed with electronic tensile machine. RESULTS AND CONCLUSION： The braided polyurethane scaffold materials had no cytotoxicity; ACL and NIH3T3 cells adhered, grew and proliferated well on the surface of the braided polyurethane scaffold materials. The Young’s modulus, ultimate tensile stress and elongation at break were （41.2±2.1） MPa, （51.0±1.5） MPa and （600±60）% respectively. The polyurethane scaffold materials possess good biomechanical properties and biological safety.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第51期9535-9538,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省自然科学基金(8151803601000013)~~
