摘要
目的模拟高原缺氧环境,构建兔牙周炎动物模型,研究其龈下菌斑中的主要牙周致病菌与平原组的差异。方法选取清洁级家兔40只完全随机设计分成4组:平原实验组、平原对照组、高原实验组、高原对照组各10只,实验组采用正畸结扎丝结扎双下颌前牙,并给予高糖饮食;高原组置模拟海拔5 000 m的低压氧舱,每天缺氧23 h,持续饲养8周。8周后提取龈下菌斑基因组DNA,用PCR扩增细菌16S rDNA片段方法检测标本中6种牙周炎致病菌包括牙龈卟啉单胞菌(Dorhyromonas gingivalis,Pg)、福赛斯坦纳菌(Bacterides forsythus,Bf)、伴放线放线杆菌(Actinobacillus actinomycetem-comitans,Aa)、中间普氏菌(Prevotella intermedia,Pi)、具核梭杆菌(Fusobacterium nucleatum,Fn)和齿垢密螺旋体(Trepone-ma denticola,Td)的检出率。结果与平原实验组相比,高原实验组的牙龈出血指数、菌斑指数和牙周袋深度均有统计学差异(P<0.05)。牙周可疑致病菌Pg、Bf、Aa、Pi、Fn和Td在高原实验组和平原实验组都可检出,其中高原实验组Fn的检出率80%高于平原实验组20%(P<0.05);平原实验组Pi的检出率70%高于高原实验组15%(P<0.05)。结论高原低氧环境是牙周炎加重的主要原因,Fn与高原牙周炎病变程度加重有一定关系。
Objective To investigate the difference of main pathogenic bacteria in subgingival plaque of periodontitis rabbits under normal oxygen and simulated high altitude hypoxia conditions. Methods A total of 40 rabbits were randomly divided into 4 groups including a normal oxygen periodontitis group, a normal control group, a hypoxia periodontitis group and a hypoxia control group （n = 10）. Rabbits of the periodontitis groups were given ligation of anterior teeth by steel wire and high-carbohydrate diet, while rabbits of the hypoxia groups were raised in a low pressure oxygen cabin simulating high altitude hypoxia condition （5 000 m） , 23 h/day for 8 weeks. Genomic DNA in subgingival plaque was extracted after 8 weeks treatment and 16S rDNA gene fragments were amplified by polymerase bacteria, comitans Results chain reaction （PCR） to test 6 periodontits pathogenic including Porphyromonas gingivalis （ Pg ） , Bacteroides forsythus （Bf） , Actinobacillus actinomycetem- （ Aa ） , Prevotella intermedia （ Pi ） , Fusobacterium nucleatum （Fn） and Treponema denticola （Td）. Gingival bleeding index, plaque index and periodontal pocket depth in the hypoxia periodontitis group were significantly different from those in the normal oxygen periodontitis group （ P 〈 0.05 ）. Putative periodontal pathogens, including Pg, Bf, Aa, Pi, Fn and Td, were detected both in the hypoxia periodontitis group and normal oxygen periodontitis group. The positive rate of Fn in the hypoxia periodontitis group （80%） was higher that in the normal oxygen periodontitis group （20%） （P 〈 0.05）, while the positive rate of Pi was higher in the normal oxygen periodontitis group （70%） than that in the hypoxia periodontitis group （15%） （ P 〈 0.05）. Conclusion High altitude hypoxia is the main reason of periodontitis aggravation, which is alsoassociated with Fn.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2012年第3期206-209,共4页
Journal of Third Military Medical University
基金
全军医学科研"十一五"计划面上项目(06MA184)~~
关键词
牙周炎
高原低氧
动物模型
致病菌
periodontitis
high altitude hypoxia
animal model
pathogenic bacteria
