摘要
目的探讨氢氧化锌与低分子量透明质酸(Low molecular weight hyaluronic acid,LHA)复合佐剂对甲肝乙肝联合抗原诱导小鼠体液免疫应答的影响。方法在甲肝乙肝联合抗原中加入不同配比的Zn(OH)2与LHA复合佐剂,经皮下免疫ICR小鼠,并设铝佐剂组、生理盐水对照组和单纯抗原组。分别于免疫后4、8、12和16周采血,分离血清,ELISA法检测小鼠血清抗-HAV IgG和抗-HBsAg IgG水平。结果除对照组小鼠血清检测不到抗-HAV IgG抗体外,其余各组小鼠血清抗-HAV IgG水平在12周时达峰值,抗-HBsAg IgG水平在8周时达峰值,以后逐渐下降;免疫后4、8、12和16周,多数复合佐剂组抗-HAV IgG和抗-HBsAg IgG水平与抗原组和铝佐剂组相比,显著增强(P<0.05),且抗体水平持续时间较长。结论适当剂量配比的氢氧化锌与LHA复合佐剂能增强甲肝乙肝联合抗原诱导的体液免疫应答。
Objective To investigate the effect of zinc hydroxide[Zn（OH）2] and low molecular weight hyaluronic acid （LHA） compound adjuvant on humoral immunity induced by hepatitis A and B combined antigen in mice. Methods Zn （OH）2/LHA compound adjuvant with various formula were added into hepatitis A and B combined antigen respectively. ICR mice were immunized s. c. with the mixture, using aluminium hydroxide adjuvant, physiological saline and adjuvant-free antigen as control. Serum samples were collected 4, 8, 12 and 16 weeks after immunization and determined for anti-HAV IgG and anti-HBsAg IgG levels by ELISA. Results Except those in physiological saline control group, the serum anti-HAV IgG and anti-HBsAg IgG levels of mice in various groups reached peak values 12 and 8 weeks after immunization respectively then decreased gradually. Most of the anti-HAV IgG and anti-HBsAg IgG levels of mice 4, 8, 12 and 16 weeks after immunization with hepatitis A and B combined antigen containing Zn（OH）J LHA compound adjuvant, at various dosages, increased significantly as compared with those in aluminium hydroxide adjuvant and adjuvant-free antigen control groups （P 〈 0. 05）, and lasted for a long time. Conclusion The Zn（OH）2/LHA compound adjuvant with appropriate formula enhanced the humoral immune response induced by hepatitis A and B combined antigen.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第1期29-31,35,共4页
Chinese Journal of Biologicals
基金
国家"艾滋病和病毒性肝炎等重大传染病防治"重大专项-新型疫苗佐剂平台研究(2009ZX10004-702)
昆明市科技计划项目"新型疫苗佐剂平台研究"(昆科计字09H100302)
关键词
氢氧化锌
低分子量透明质酸
复合佐剂
甲乙肝联合抗原
体液免疫
Zinc hydroxide Low molecular weight hyaluronic acid Compound adjuvant Hepatitis A and B combinedantigen Humoral immunity
