摘要
为了探讨牛乳房炎的分子机制,试验采用RT-PCR和测序相结合的方法克隆了牛TIRAP基因的编码区序列,并采用生物信息学方法分析了TIRAP基因及其所编码蛋白质的结构和功能。结果表明:获得TIRAP基因的cDNA序列,其1～699 bp为编码区(CDS区),编码232个氨基酸,其蛋白质的分子质量为24 433.5 u,等电点为6.95,含有TIR结构域(95～179aa),位于细胞核、细胞质、线粒体、细胞骨架等多种细胞器上;该蛋白与人、小鼠、大鼠和狗蛋白的同源性分别为76%、65%、64%和76%,TIR结构域的同源性均达到85%左右。结合人、小鼠TIRAP在TLRs信号转导中的重要作用,说明牛TIRAP蛋白可作为牛TLRs信号转导中的重要信息分子,在乳房炎等受病原体感染所致疾病抗性的分子机制中发挥重要功能。
To clarify the molecular mechanism of bovine mastitis,the coding region sequences of bovine TIRAP gene were isolated by RT-PCR and the sequencing,and its structure and function were analyzed by bio-informatics method.The results showed that partly cDNA sequence were isolated,which included complete CDS（1~699 bp）,and it encoded 232 amino acid.The molecular weight and isoelectric point of TIRAP protein were 24 433.5 u and 6.95,respectively.TIRAP protein contained TIR domains（95~179 aa）,locating on many cell organs,such as cell nucleus,cytoplasm,mitochondria and cytoskeleton.The homologies of TIRAP protein among cattle,human,mouse,rats and dogs were 76%,65%,64% and 76%,respectively.The homologies of TIR domain of TIRAP protein among all species as mentioned above were 85%.According to the results in this study and the function of TIR domain of TIRAP protein in human and mouse,it infers that TIRAP may be an important signaling molecule for TLRs signal transduction,and may be very important for the disease caused by pathogenic infection,such as mastitis in diary cattle.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2011年第12期20-22,共3页
Heilongjiang Animal Science And veterinary Medicine
基金
湖南省科技计划项目(2010NK3043)
湖南省自然科学基金项目(11JJ4020)
湖南省高校创新平台开放基金项目(11K045)
湖南文理学院博士启动项目(BSQD1002)
