摘要
为了能够更加准确的进行动物源性成分定量检测,打击市场上的"掺杂使假"行为,本实验从初始取样量、裂解时间、沉淀时间和RNA酶作用四方面对CTAB法提取肉类DNA进行研究。综合考虑方法的快速、高效性,最终确定初始取样量应该小于0.05g,裂解时间和沉淀时间均为0.5h,不需添加RNA酶。本方法具有良好的线性关系(R2=0.9972)和稳定性(标准差为0.18),是一种快速、准确、规范的肉类总DNA提取方法。
An optimized CTAB method for total RNA extraction was presented for use in accurately quantifying components of animal origin and fighting against adulterated food in the market as a sample preparation method. The use of the CTAB method to extract total RNA from pork was investigated with respect to sample amount, dissociation time, precipitation time and RNase treatment. As a result, a fast and efficient method for total RNA extraction was achieved by selecting sample amount below 0.05 g, 0.5 h dissociation, 0.5h precipitation and non-RNase treatment. The method showed a good linear relationship （R2 = 0.9972） and stability （SD = 0.18）.
出处
《肉类研究》
2011年第12期22-24,共3页
Meat Research
基金
国家"863"计划项目(2011AA100805)
北京市科技计划项目(D101110047710003)
