摘要
目的:探讨L型结核分枝杆菌rpoB基因突变与利福平耐药性的关系。方法:采用PCR和PCR-DS技术对23例初治肺结核和45例复治肺结核患者L型结核分枝杆菌临床分离株进行rpoB基因检测和序列分析。结果:初治肺结核L型结核分枝杆菌rpoB基因突变率为4.3%,复治肺结核耐L型结核分枝杆菌rpoB基因突变率为40.0%。结论:复治肺结核L型结核分枝杆菌rpoB基因突变率高于初治肺结核,L型菌rpoB基因突变是造成结核分枝杆菌形成利福平耐药性的主要机制。
Objective： To study the relationship of gene mutation and rifampin resistance in L-forms of mycobacterium tuberculosis.Methods： rpoB genes mutation clinical isolated strains of mycobacterial L forms in 23 the first-treated patients and 45 second-treated patients were detected by PCR DS.Results： The rpoB gene mutation rate of first-treated patients and second-treated patients were 4.3% and 40.0% respectively.Conclusion： The rpoB genes mutation clinical isolated strains of mycobacterial L forms in second-treated patients was higher than that in first-treated patients and it was considered an important rifampin resistant mechanism of mycobacterium tuberculosis.
出处
《南通大学学报（医学版）》
2011年第6期433-434,共2页
Journal of Nantong University(Medical sciences)
基金
南通市社会发展项目(S2009023)
关键词
结核分枝杆菌
细胞壁缺陷
RPOB基因
耐药性
mycobacterium tuberculosis
cell wall deficient
rpoB gene
drug resistance
