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实时荧光定量PCR法检测呼吸系统感染儿童肺炎支原体DNA的分析 被引量:3

DNA Detection of Pneumonia Mycoplasma by Fluorescent Quantitative PCR Method in Children with Respiratory Infection
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摘要 目的:了解呼吸道感染患儿肺炎支原体、解脲脲原体、沙眼衣原体的感染情况。方法:应用荧光定量聚合链式反应法对124例呼吸道感染患儿同时进行肺炎支原体、解脲脲原体、沙眼衣原体检测。结果:肺炎支原体、解脲脲原体、沙眼衣原体总检出率为52.0%,其中肺炎支原体为24.0%,解脲脲原体为21.3%,沙眼衣原体为10.2%,肺炎支原体和解脲脲原体为2.2%,解脲脲原体和沙眼衣原体为2.4%。在年龄分布上,〈28 d组患儿解脲脲原体感染率高达30.3%,且与其他组的差异有统计学意义,1个月~1岁组、〉1~3岁组和〉3岁组的患儿肺炎支原体感染率明显高于〈28 d组,差异有统计学意义。肺炎支原体感染率四季差异无统计学意义,解脲脲原体冬春季感染率高,沙眼衣原体春季感染率低。结论:新生儿较其他年龄段患儿易感解脲脲原体、沙眼衣原体;肺炎支原体随着年龄的增长感染率逐渐增高。荧光定量聚合链式反应法诊断支原体、衣原体感染快速、敏感,特异性高。 Objective:To investigate the situation of infection of mycoplasma pneumoniae(MP),Ureaplama urealyticum(Uu) and Chtamydia trachomatis(CT) in children with respiratory infection.Methods:MP,Uu and CT were measured by fluorogenic quantitative PCR in 124 children with respiratory infection.Results:Total infectious rate of MP,Uu and CT was 52.0%,in which Mp was 24.0%,Uu was 21.3%,CT was 10.2%,MP with Uu was 2.2%,Uu with CT was 2.4%.In the age distribution,the infectious rate of Uu in the(28 days)group was higher(30.3%)than other groups(P 0.05).The infectious rate of Mp in the groups(1months to 1 years old /1 years old to 3 years old/over 3 years old) was higher than in the(28 days) group.The infectious rate of MP was not significant different between the four seasons(P0.05).The infectious rate of Uu was high in winter and spring,and the infectious rate of CT was low in spring.Conclusion:Neonates demonstrate a marked susceptibility to Uu and CT infections;Infectious rate of MP is increased with age.Fluorogenic quantitative PCR is sensitive,specific and rapid to diagnosis of MP,Uu and CT infections.
出处 《内蒙古医学杂志》 2011年第10期1190-1192,共3页 Inner Mongolia Medical Journal
关键词 呼吸道感染 肺炎支原体 Respiratory tract infection Mycoplasma pneumoniae
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