摘要
[目的]观察中药正肝方药物血清对甲胎蛋白(AFP)刺激的AFPsiRNA-HepG2细胞增殖的影响。[方法]正肝方浸膏粉经灌胃给药制备正肝方大鼠血清,体外培养AFPsiRNA-HepG2细胞,将细胞分为5%正常大鼠血清、5%正肝方血清、10%正常大鼠血清、10%正肝方血清、5%正常大鼠血清加AFP刺激、5%正肝方血清加AFP刺激、10%正常大鼠血清加AFP刺激、10%正肝方血清加AFP刺激共8组,MTT法检测细胞增殖。[结果]无AFP刺激时,正肝方血清组与正常大鼠血清组OD值差异无统计学意义(P>0.05);有AFP刺激时,正肝方血清组与正常大鼠血清组OD值差异有统计学意义(P<0.01),正肝方血清组显著抑制AFPsiRNA-HepG2细胞增殖。[结论]正肝方血清通过影响AFP介导的AFPsiRNA-HepG2细胞的增殖抑制肝癌细胞增殖。
[Objective]To observe the effect of Zhenggan recipe（ZR） on the proliferation of AFPsiRNA-HepG2 cells stimulated by AFP.[Methods]The drug serum was collected from rats.AFPsiRNA-HepG2 cells were cultured with drug serum and stimulated by AFP or serum without AFP.The proliferation was evaluated by MTT assay.[Results]Without AFP,there were no significant difference between normal group and drug serum group.With AFP,the drug serum markedly inhibited the proliferation of AFPsiRNA-HepG2 cells compared with normal group.[Conclusion]ZR could inhibit the proliferation of hepatocarcinoma cells via influencing the proliferation pathway of AFPsiRNA-HepG2 cells mediated by AFP.
出处
《中国中西医结合消化杂志》
CAS
2011年第6期351-353,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
国家自然科学基金资助项目(30873348)
深圳市科技局资助项目(200902100)
