摘要
目的应用环介导等温扩增法(LAMP)对肺炎患者下呼吸道分泌物进行11种常见致病菌的核酸检测,探讨其临床应用价值。方法收集福建省立医院2009—2010年呼吸科诊断为下呼吸道感染患者的合格痰标本289例,行LAMP方法检测,与痰培养结果比较,分析其灵敏性和特异性。结果 LAMP检测时间仅需2～3h,明显短于痰培养;以细菌浓度>1×103为阳性阈值,敏感性74.0%,特异性95.3%,敏感性明显高于痰培养;其阳性率为42.9%(124/289)。结论 LAMP法可方便、快速检测肺部感染的常见病原菌,敏感性高于痰培养法。
Objective To detect nucleic acids of 11 common pathogenic bacteria in lower respiratory tract from pneumonia patients by loop-mediated isothermal amplification（LAMP） and evaluate its clinical application.Methods Qualified sputum samples from 289 patients with lower respiratory infections were collected during 2009-2010 in Fujian provincial hospital and were detected by LAMP method.Comparing with routine sputum culture,the sensitivity and specificity of LAMP method were analyzed.Results It took 2~3 hours only for one LAMP assay,significantly shorter than that for sputum culture method.With1×103 copies of bacteria as a positive threshold,the sensitivity was 74.0% and specificity 95.3%.The sensitivity for LAMP was significantly higher than sputum culture.The positive rate was 42.9%（124/289）.Conclusion LAMP method is a convenient and rapid assay for detecting common pathogens of lower respiratory tract infections.The sensitivity of LAMP were higher than that of conventional culture method for clinical diagnosis.
出处
《海峡预防医学杂志》
CAS
2011年第6期1-2,17,共3页
Strait Journal of Preventive Medicine
基金
福建省科技厅科技计划项目(No.2009Y0010)
福建省卫生厅医学创新课题(No.2009CXB-2)
关键词
病原检测
常见病原菌
环介导等温扩增方法
下呼吸道感染
临床应用
Pathogen test
Common Pathogens
Loop-mediated Isothermal Amplification（LAMP）
Lower Respiratory Tract Infection
Clinical Application
