摘要
目的:研究骨钙素在使用BMP2/7异源二聚体促进种植体周骨缺损再生中的表达。方法:建立小型猪种植体周骨缺损模型,并使用BMP2/7异源二聚体及BMP2、BMP7同源二聚体促进骨再生,采用免疫组化方法分别在2、3、6周时检测新骨中骨钙素表达。结果:在各实验组中,骨钙素3周时表达达到最高值。在2、3、6周,BMP2/7组骨钙素表达均强于BMP2、BMP7同源二聚体组及对照组。结论:在以同样较低浓度(30ng/ml)作用于小型猪种植体周骨缺损诱导新骨形成过程中,BMP2/7异源二聚体较BMP、BMP7同源二聚体有效促进骨钙素的表达。
Objectives： To delineate expression of osteocalcin induced by recombinant human bone morphogenetic protein（BMP）2/7 heterodimer in peri-implant bone defects in comparison to BMP2 and BMP7 homodimer.Methods： Identical peri-implant bone defects were created on the frontal skull of minipig.Collagen sponges with the same dose BMP2/7 heterodimer、BMP2 homodimer 、BMP7 homodimer 、or BMPs were adopted to treat the freshly created implant bed.Titanium implants were centrally implanted with 4mm-fixture within bone defects.Immunohistochemical method was applied to evaluate the expression of osteocalcin（OCN） after 2,3,and 6 weeks of implantation.Results： The OCN proteins displayed intensity peaks at 3 weeks（P0.05） in all the groups.Immunoreactive expression of OCN of BMP2/7 group was more intensity than BMP2、BMP7 and control group at 2,3,and 6 weeks post-operation（P0.05）.Conclusions： Purified recombinant human BMP2/7 heterodimer can enhance the expression of OCN with significant differences over BMP2 homodimers or BMP7 homodimers with the same low-dose（30ng/ml）.
出处
《中华老年口腔医学杂志》
2011年第4期210-214,共5页
Chinese Journal of Geriatric Dentistry
基金
浙江省自然科学基金(项目编号:Y2101440)
浙江省教育厅科研基金(项目编号:Y200908995)
关键词
种植体
骨钙素
BMP
异源二聚体
同源二聚体
Bone morphogenetic protein
Heterodimer
Homodimer
Implant osteocalcin
