摘要
以冬虫夏草新鲜子实体为材料,分别采用SDS抽提法、TRIzol法、CTAB-LiCl法和DEPC水法4种方法进行总RNA的提取试验,并对其进行了紫外及变性琼脂糖凝胶电泳检测。检测结果表明,4种提取方法中DEPC水法提取的总RNA质量最好,SDS抽提法次之,CTAB-LiCl法和TRIzol法效果最差。从RNA质量、操作难度、经济与否等方面综合分析,DEPC水法是较为理想的提取冬虫夏草新鲜子实体总RNA的方法。
Taking the fruit-body of Cordyceps sinensis as material, the comparative analysis of the four total RNA extraction methods were carried out, including SDS extraction method, TRIzol method, CTAB-LiCl method and DEPC water method, and the denaturing agarose gel electrophoresis was also carried out. The results showed that the purity and integrality of the extracted total RNA by the four methods was as follows： DEPC water method〉SDS extraction method〉CTAB-LiCl method〉 TRIzol method. Considering the RNA quality, operation difficulty and economical efficiency comprehensively, the DEPC water method was the best one for extracting total RNA from C. sinensis.
出处
《长江蔬菜》
2012年第2期19-21,共3页
Journal of Changjiang Vegetables
基金
陕西省教育厅重点试验室项目(2010JS065)
延安市科学技术研究发展计划项目(2010kn-03)
延安大学专项科研基金项目共同资助
关键词
冬虫夏草
总RNA
提取方法
Cordyeeps sinensis
Total RNA
Extraction method
