摘要
目的:建立测定紫杉醇脂质纳米粒体外释放度的方法,考察紫杉醇脂质纳米粒24h的体外释放度。方法:采用固相萃取-高效液相色谱法。样品经C18固相萃取柱萃取,采用Phenomenex luna C18(250mm×4.6mm,5μm)色谱柱分离,流动相为甲醇-水(80:20),流速为1.0mL.min-1,检测波长为227nm。结果:紫杉醇的检测浓度在2～12μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9993),紫杉醇脂质纳米粒在水杨酸钠(1mol.L-1)-PBS(pH7.4)中24h累积释放度为104.9%,其释放规律符合一级动力方程。结论:所建方法简便、灵敏,干扰小,可用于体外释放的检测。
OBJECTIVE： To develop a solid phase extraction （SPE）-HPLC method for in vitro drug release of Paclitaxel lipid nanoparticles, and to investigate in vitro drug release of Paclitaxel lipid nanoparticles within 24 h. METHODS： The sample was purified with C18 solid phase extraction columns. The chromatographic analysis was performed on a phenomenex luna C18（250 mm×4.6 mm, 5 μm） column with mobile phase consisted of methanol-water （80：20） at the flow rate of 1.0 mL.min-1. The detection wavelength was set at 227 nm. RESULTS： The linear range of paclitaxel was 2-12 μg.mL-Z（r=0.999 3）. The in vitro cumulative release of paclitaxel in 1 mol. L-1 sodium salicylate solution -PH 7.4 PBS reached about 104.9% in 24 h. The profile was described by first-class equation. CONCLUSION： The method is simple, sensitive and little interference, and it is suitable for the determination of in vitro drug release of sample.
出处
《中国药房》
CAS
CSCD
2012年第7期608-610,共3页
China Pharmacy
