摘要
目的:探讨正常口腔黏膜上皮组织、口腔鳞状细胞癌(OSCC)癌旁组织及OSCC组织中CD44的表达强度,为确定CD44作为OSCC基因治疗的参考指标提供依据。方法:应用免疫组织化学SP法检测65例OSCC患者OSCC组织、20例OSCC患者OSCC癌旁组织和10例正常口腔黏膜上皮组织中CD44的表达强度。结果:CD44在OSCC组织中的阳性表达率高于其在正常口腔黏膜上皮组织及OSCC癌旁组织中的阳性表达率(P<0.05);CD44表达率随肿瘤分化程度降低而增高;CD44在有淋巴结转移的OSCC组织中的表达率高于无淋巴结转移组(P<0.05);CD44在临床分期为晚期的OSCC组织中表达高于早期(P<0.05);CD44表达率与患者的年龄、性别、肿瘤大小及部位无明显关联。结论:CD44在正常口腔黏膜上皮组织、OSCC癌旁组织和OSCC组织中表达强度不同,提示CD44的高表达可作为判定OSCC的恶性程度及预后的重要指标。
Objective To investigate the expressions of CD44 in normal oral mucosa tissues,paracancerous tissues and oral squamous cell carcinoma（OSCC）tissuses and to provide the basis for the determination of CD44 in gene therapy of OSCC.Methods The expression of CD44 in 65 cases of OSCC,20 cases of paracancerous tissues and 10 cases of normal oral mucosa tissues were detected by immunohistochemistry method.Results The positive expression rate of CD44 in OSCC tissues was higher than those in normal oral mucosa and paracancerous tissues（P0.05）.The expression rate of CD44 was increased with the decreasing of the differentiation degree of OSCC.The expression of CD44 was higher in the cases with lymphnode metastasis than that in the cases without lymph node metastasis（P0.01）.The expression of CD44 in advanced stage group was higher than that in early stage group which were classified by clinical stages（P0.05）.The expression of CD44 was not related to the gender,age of the patients or the length,localization of carcinoma（P0.05）.Conclusion The expression levels of CD44 in normal oral mucosa tissues,paracancerous tissues and OSCC tissues are different,it indicates that high expression of CD44 may be an important indicator for judgement of malignant degree and prognosis of OSCC.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2012年第1期106-109,I0002,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科研基金资助课题(200705343)
关键词
CD44
口腔鳞状细胞肿瘤
免疫组织化学
HE染色
CD44
oral squamous cell carcinoma
immunohistochemistry
hematoxylin eosin staining
