摘要
用反相高效液相色谱法测定了槐花中芦丁的含量。采用μBondapakC18柱,甲醇-水-冰乙酸(40∶575∶25)为流动相,检测波长254nm。试验表明,线性关系良好(r=09996),平均回收率达9811%。该测定方法简便、准确,重现性好。
A RP-HPLC method was established for the determination of Rutin in Flower Bud of Japanese Pagodatree. The μ-Bondapak C 18 column was used. The mobile phase consisted of methanol- water-glacial acetic acid (40∶57 5∶2 5). The detected wave length was 254nm. The results shown that the linearity was good (r=0 9996). The mean recovery of Rutin was 98 11%. This method is simple, accurate and precise.
出处
《中国药事》
CAS
2000年第1期34-35,共2页
Chinese Pharmaceutical Affairs
