摘要
采用电镜技术研究了两组饲料——饲料A(添加6%鳕鱼肝油和4%大豆卵磷脂,饲料含4.53%磷脂和1.08%HUFA)、饲料B(添加10%猪油,饲料含0.39%磷脂和0.18%HUFA)对两种规格(2.4 g和15.0 g)中华绒螯蟹幼蟹肝胰腺超微结构的影响,结果表明:A组饲料两种规格幼蟹肝胰腺R细胞可见有脂肪滴,顶端微绒毛排列整齐,线粒体多;B组饲料两个时期幼蟹肝胰腺许多细胞解体,R细胞内脂肪滴十分充盈,顶端微绒毛脱落,线粒体很少,并且,在A组饲料2.4 g幼蟹雌体R细胞内观察到许多钙质体,而A组饲料15.0 g幼蟹肝胰腺细胞核中见到形状不一的核仁.
This study is conducted to determine effects of different lipid nutrition on the hepatopancreas micro-uhrastruc- ture of juvenile Chinese mitten crab, Eriocheir sinensis. Two diets are formulated to contain different amounts of fish oil, soy lecithin and pork lard with resulting PL/HUFA levels （% dry weight） of 4.53/1.08（Diet A） and 0. 39/0.18（Diet B）, and two kinds of juvenile crabs of weight 2.4 g and 15.0 g are selected and divided into AF（Diet A , female） group, AM（Diet A, male） group, BF（Diet B, female） group and BM（Diet B, male） group, repectively. The hepatopancreas micro-ultrastructure of the juvenile crabs show lipid droplets of R cell from the crabs fed Diet B is more abundant in lipid contained than those fed Diet A, fewer mitochondria are found in the R cell from the crabs fed Diet B, and the microvilli of a lot of R cells from the crabs fed Diet B have disappeared. Interestingly, much Ca is found in the R cell from the juvenile of weight 2.4 g females fed Diet A, and a ruleless figure nucleolus is found in nucleus of R cell from crabs of weight 15.0 g fed Diet A.
出处
《河南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第4期108-111,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
国家自然科学基金(30871927)
上海市科技兴农推广项目(沪农科推字2009D-2-1)
国家科技成果转化项目(2009GB2C300156)
关键词
中华绒螯蟹
磷脂
HUFA
肝胰腺
超微结构
Eriocheir sinensis
crab
PL
HUFA
hepatopancreas
micro-ultrastructure
