摘要
[目的]探讨从芹菜叶黄酮类成分的最佳工艺,为芹菜叶的开发利用提供理论依据。[方法]以乙醇为提取溶剂,采用索式抽提法从芹菜叶中提取黄酮类物质成分,通过单因素试验研究乙醇浓度、提取时间、提取温度和料液比对总黄酮得率的影响。通过正交试验优选最佳提取工艺。[结果]影响黄酮得率的因素主次顺序为:乙醇浓度>料液比>提取温度>提取时间。芹菜叶中总黄酮的最佳提取工艺为:提取溶剂乙醇的浓度为60%,提取时间为1 h,提取温度为70℃,料液比为1∶60。在此工艺条件下,提取的总黄酮得率为47.85mg/g。[结论]索式抽提法提取芹菜叶中的黄酮是一种切实可行的方法。
[Objective] To investigate the optimum extraction technology of total flavonoids from celery leaves by using Soxhlet extraction method,so as to provide a theoretical basis for the development and utilization of celery leaves.[Method] With the ethanol as the solvent,Soxhlet method was adopted to extract total flavonoids from celery leaves.Effects of ethanol concentration,extraction time,extraction temperature and solid-liquid ratio on the yield of total flavonoids were respectively investigated through the single-factor test.[Result] Factors that affected the yield of total flavonoids were in the order of ethanol concentration solid-liquid ratio extraction temperature extraction time.The optimum technology conditions of total flavonoids from celery leaves by using Soxhlet extraction method were as follows：ethanol concentration of 60%,extraction time of 1 h,extraction temperature of 70 ℃ and solid-liquid ratio of 1∶60.Under the optimum condition,the yield of total flavonoids was 47.85 mg/g.[Conclusion] The Soxhlet extraction method was available for extracting total flavonoids from celery leaves.
出处
《安徽农业科学》
CAS
北大核心
2011年第22期13409-13411,共3页
Journal of Anhui Agricultural Sciences
关键词
芹菜叶
总黄酮
提取工艺
Celery leaves
Total flavonoids
Extraction technology
