期刊文献+

牦牛源多杀性巴氏杆菌血清型鉴定及ompH基因序列分析 被引量:1

Identified the serotype and sequences analysis of the yak ompH gene of Pasteurellosis multocida
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摘要 为分析牦牛多杀性巴氏杆菌(P.multocida)分离株与疫苗株的血清型和ompH基因差异,本研究利用PCR方法鉴定其血清型,并克隆、测序ompH全长基因,用DNAMAN、DNAStar对其进行生物信息学分析。结果显示,分离株与疫苗株血清型为B型,标准株血清型为E型。分离株和疫苗株ompH基因编码氨基酸有2个缺失和2个突变,同源性为99.4%。研究表明ompH基因具有很高的同源性,但氨基酸的缺失和突变可能使抗原表位发生变化,也是导致免疫失败的原因之一。 To analyze the difference in serotype and ompH gene between clinical isolate and vaccine strain of Pastcurcllosismultocida from yak, the serotype were identified by PCR and the ompH gene were amplified for sequencing analysis. The results showed that the P. multocida isolate and vaccine was serotype B, standard strain was serotype E. Compared with vaccine slrain, the ompH ORF of the isolate shared 99.4% identity, but had 6 bp consistent deletion and two point mutation, resulting two aminoacid deletion and mutations, respectively, which might be lead to the change antigen epitopes and responsible lbr breakthrough of the vaccine immunization.
出处 《中国预防兽医学报》 CAS CSCD 北大核心 2012年第2期152-154,共3页 Chinese Journal of Preventive Veterinary Medicine
基金 新疆农垦科学院科技引导计划(YYD201107),新疆农垦科学院青年基金(YQJ2009-02、YQJ201110)
关键词 牦牛 多杀性巴氏杆菌 血清型 ompH基因 序列分析 yak Pasteurellosismultocida srotype ompH gene sequence analysis
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参考文献9

  • 1Kirsty M T,Alan J F,Chiang W L,et al.Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates[J].J Clin Microbiol,1998,36(4):1096-1100.
  • 2吴斌,唐先春,陈焕春,王大鹏.猪源多杀性巴氏杆菌ompH基因的克隆、表达[J].畜牧兽医学报,2005,36(7):701-704. 被引量:11
  • 3康立超,田亮,张晓宇,王平福,薄新文,马勋.新疆牦牛多杀性巴氏杆菌的分离鉴定及药物敏感实验[J].石河子大学学报(自然科学版),2010,28(4):436-440. 被引量:6
  • 4Kirsty M T,John D B,Jing Y C,et al.Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system[J].J Clin Microbiol,2001,39(3):924-929.
  • 5Yugang L,John R G,Mark W J,et al.Cloning and chracterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73[J].J Bacterio,1997,179 (6):7856-7864.
  • 6Lee J,Kim Y B,Kwon M.Outer membrane protein H for protective immunity against Pasteurella multocida[J].J Microbiol,2007,45:179-184.
  • 7John M N,Kyungcheol Y,Richard J K,et al.Antibodies to loop 6 of the P2 porin protein of nontypeable Haemophilus influenzae are bactericidal against multiple strains[J].Infect Immunity,2001,69:773-778.
  • 8Kyungcheol Y,Timothy F M.Importance of an immunodomiuant surface-exposed loop on outer membrane protein P2 of nontypeable Haemophilus influenzae[J].Infect Immun,1997,65:150-155.
  • 9Robert L D,Roslyn M,Susan B,et al.Characterization and comparison of pasteurella multocida strains associatedwith porcine pneumonia and atrophic rhinitis[J].J Med Microbiol,2003,52:59-67.

二级参考文献18

  • 1沈丹,黄青云.禽大肠杆菌巴氏杆菌融合二联弱毒菌株F_4安全性免疫原性研究[J].畜牧兽医学报,2004,35(6):675-679. 被引量:3
  • 2唐先春,吴斌,索绪峰,王大林,陈焕春,尹争艳.猪多杀性巴氏杆菌的分离鉴定及生物学特性研究[J].畜牧兽医学报,2005,36(6):590-595. 被引量:48
  • 3郭明璋,王启明,董亚芳,薛家宾,徐为中.ELISA检测兔巴氏杆菌病抗体的研究[J].中国兽药杂志,1997,31(1):1-5. 被引量:7
  • 4吴范庚 钱心元.我国多杀性巴氏杆菌耐热抗原血.中国兽医杂志,1987,13(12):2-4.
  • 5陈薄言.兽医传染病学[M].北京:中国农业出版社,2007.
  • 6Townsend K M,Frost A J,Lee C W,et al.Development of PCR assays for species and type-specific identification of Pasteurella multocida isolates[J].Journal of Clinical Microbiology,1998,36(4):1096-1100.
  • 7Kirsty M Townsend,John D Boyce,Jing Y Chung,et al.Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system[J].Journal of Clinical Microbiology,2001,39(3):924-929.
  • 8郝士海.现代细菌学培养基和生化试验手册[M].北京:中国科学技术出版社,1992.266-273.
  • 9萨姆布鲁克 J, 弗里奇 E F, 曼尼阿蒂斯 T. 分子克隆实验指南[M]. 金冬雁, 黎孟枫,侯云德,等译.第2版. 北京:科学出版社, 1996.
  • 10Chevalier G, Duclohier H, Thomas D, et al. Purification and characterization of protein H, the major porin of Pasteurella multocida[J]. J Bacteriol, 1993, 175: 266~276.

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