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Extraction, purification and antioxidant activity of polysaccharides from bamboo leaves 被引量:12

Extraction, purification and antioxidant activity of polysaccharides from bamboo leaves
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摘要 Ultrasonic extraction (UE) was employed for the extraction of bamboo leaf polysaccharides (BLP). The influential parameters of UE procedure including extraction time, ultrasonic power and solid/liquid ratio were optimized by orthogonal experiments. DEAE-cellulose col- umn chromatography was applied to purify BLP and then the radical scavenging activity of BLP was also evaluated. Optimal extraction con- ditions were: extraction time .of 15 min, ultrasonic power of 300 W, and solid/liquid ratio of 1:15. Four kinds of polysaccharides were obtained by DEAE-cellulose colunm chromatography; the maximum superoxide radical scavenging rate (20.4%) of BLP was inferior to that of vitamin C (Vc, the control) and the hydroxyl radical scavenging rate (50%) was equivalent to that of Vc. Ultrasonic extraction (UE) was employed for the extraction of bamboo leaf polysaccharides (BLP). The influential parameters of UE procedure including extraction time, ultrasonic power and solid/liquid ratio were optimized by orthogonal experiments. DEAE-cellulose col- umn chromatography was applied to purify BLP and then the radical scavenging activity of BLP was also evaluated. Optimal extraction con- ditions were: extraction time .of 15 min, ultrasonic power of 300 W, and solid/liquid ratio of 1:15. Four kinds of polysaccharides were obtained by DEAE-cellulose colunm chromatography; the maximum superoxide radical scavenging rate (20.4%) of BLP was inferior to that of vitamin C (Vc, the control) and the hydroxyl radical scavenging rate (50%) was equivalent to that of Vc.
出处 《Journal of Forestry Research》 SCIE CAS CSCD 2012年第1期139-143,共5页 林业研究(英文版)
关键词 bamboo leaves POLYSACCHARIDE scavenging radical separa-tion and purification ultrasonic extraction bamboo leaves polysaccharide scavenging radical separa-tion and purification ultrasonic extraction
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