摘要
通过空间诱变从光身稻品种Francis的M2群体中发现1株叶色白化转绿、多分蘖矮秆突变体hfa-1。hfa-1在三叶期之前完全白化,随后转绿。白化转绿表型受生长发育和温度调控。亚细胞结构观察发现hfa-1叶绿体发育异常抑制叶绿素合成,造成光合效率降低,产生白化表型。hfa-1的多分蘖表型是由于高节位分蘖芽激活所致,初步鉴定与苗期叶片IAA(吲哚乙酸)含量无关。hfa-1的矮生性则由节间长度缩短所致,与苗期GA(赤霉素)的合成和信号传导无关。遗传分析表明hfa-1的白化转绿、多分蘖矮秆表型受单隐性核基因hw-1(t)控制。利用hfa-1与粳稻品种02428杂交获得的F2群体将hw-1(t)定位在水稻第4染色体长臂上两个InDel标记HW27和HW7间46.9kb的物理距离内,该区域有13个阅读框架,其中LOC_Os04g57320编码IMMUTANTS蛋白,推测为hw-1(t)的候选基因。
A rice（Oryza sativa L.） mutant hfa-1 exhibiting green-revertible albino,high-tillering dwarf was detected from a M2 population of an American variety Francis by space mutagenesis.hfa-1 displayed distinctive albino before 3rd leaf stage but fi-nally turned to normal green after 3rd leaf stage,and the expression of this phenotype was conditionally controlled by develop-ment and temperature.Examining ultrastructure and measuring chlorophyll content indicated that hfa-1 showed albino due to abnormal development of chloroplast,which resulted in inhibiting chlorophyll synthesis and decreasing photosynthetic efficiency.Moreover,the increased tiller number of hfa-1 was ascribed to initiate more higher-order tiller buds.Further endogenous hor-mones analysis demonstrated that the enhanced tillering capacity of hfa-1 might not result from inhibiting the synthesis of IAA.In addition,the dwarfism of hfa-1 was caused by shortening internodes and was independent of biosynthesis and signal transduction of GA.Genetic analysis indicated that the phenotype of green-revertible albino,high-tillering dwarf in hfa-1 was controlled by a recessive nucleic gene,namely hw-1（t）.Using a large F2 mapping population derived from a cross between hfa-1 and an japonica rice variety,02428,hw-1（t） was fine mapped into a 46.9 kb of physical distance between two InDel markers,HW27 and HW7 on chromosome 4,where 13 open reading frames were predicted.In the mapping interval LOC_Os04g57320 encoded a IMMUTANTS protein,which was the most properly candidate gene of hw-1（t）.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第1期23-35,共13页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30771313)
国家科技支撑计划项目(2008BAD97B02)资助
关键词
水稻
白化转绿
多分蘖矮秆
hw-1(t)
精细定位
Rice（Oryza sativa L.）
Green-revertible albino
High-tillering dwarf
hw-1（t）
Fine mapping
