摘要
[Objective] The paper aimed to study the tissue culture and transformation of C. equisetifolia. [Method] C. equisetifolia were used as experimental materials to explore the effects of three conditions including callus induction, adventitious bud dif- ferentiation and Agrobacterium-mediated transformation on transformation rate of C. equisetifolia. [Result] The appropriate plant growth regulator combination on induction and differentiation of C. equisetifolia adventitious buds was DCR＋5.0 mg/L of 6-BA＋ 0.5 mg/L of NAA; hygromycin was selected for the selective pressure and co-culture time was 3 d; 94 stains of transgenic C. equisetifolia were obtained with the initially- established transgene system via Agrobacterium-mediated method, and 61 stains were PCR-positive plants according to the results of PCR detection. [Conclusion] The study had laid the foundation for tissue culture and transgene research of C. equi- setifolia.
[目的]对细枝木麻黄进行组织培养和转基因研究。[方法]以耐寒性细枝木麻黄为试验材料,探讨了愈伤组织诱导、不定芽分化、农杆菌介导转化3种条件对转化效率的影响。[结果]对细枝木麻黄不定芽诱导分化适宜的激素组合为DCR+6-BA5.0mg/L+NAA0.5mg/L;转基因抗生素选择压力为潮霉素,共培养时间3d;以初步建立的转基因体系利用农杆菌介导法获得94株转基因木麻黄,通过PCR检测,其中61株为PCR阳性植株。[结论]该研究为细枝木麻黄的组织培养和转基因研究奠定了基础。
基金
Supported by Zhejiang Provincial Natural Science Foundation(Y306072)
浙江省自然科学基金项目(Y306072)资助
