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HFJ和Wistar大鼠脂肪肝及胰岛素抵抗动物模型的比较 被引量:1

Comparison of the Fatty Liver and Insulin Resistance Animal Model in HFJ Inbred Rats and Wistar Closed Colony Rats
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摘要 目的建立近交系HFJ大鼠和封闭群Wistar大鼠脂肪肝胰岛素抵抗动物模型及比较其生物学特性。方法雄性HFJ大鼠和Wistar大鼠,分别随机分为模型组和正常组,模型组喂养高脂饮食,正常组喂养普通饮食,两组脂肪分别占摄入能量的44.2%和19.2%,共饲养12周。每周称体质量,测定血糖、甘油三酯、胆固醇、ALT、AST、HDLC、LDLC和血胰岛素水平。实验期末处死动物摘取肝脏并称质量,计算肝指数;鼠肝脏用10%甲醛固定,石蜡包埋切片,HE染色,光镜下评估肝脂肪变性和炎症活动情况。结果镜下可见,HFJ和Wistar大鼠模型组肝细胞均呈现弥漫性脂肪变性,小叶内可见炎症细胞浸润,HFJ比Wistar脂肪变性较重,对照组肝脏均未见异常。两种动物的模型组ALT、AST、肝指数、HOMA-IR指数均显著高于其正常组,HFJ和Wistar种系间各指标的差异无显著性;HFJ大鼠模型组体重和正常组体重具有显著性差异(P<0.01),而Wistar大鼠模型组体重与正常组体重间无显著差异(P>0.05);HFJ大鼠TG和TC含量均显著高于Wistar大鼠。结论通过高脂饮食喂养成功建立了HFJ大鼠和Wistar大鼠脂肪肝与胰岛素抵抗疾病动物模型,与Wistar大鼠比较,HFJ大鼠具有自发性高血脂特征,造模更易成功,可为胰岛素抵抗和脂肪肝的发病机制研究、防治高血脂药物筛选提供一种新的实验动物。 Objective To establish fatty liver and insulin resistance animal model in HFJ inbred rats and Wistar closed colony rats,and compare their biological characteristics.Methods Male HFJ rats and Wistar rats were randomly divided into model group and control group,which were fed with high-fat diet(44.2% of energy from fat) and standard diet(19.2% of energy from fat) respectively for 12 weeks.All rats were weighed every week.Fasting blood glucose,alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),total cholesterol(TC),HDLC,LDLC and fast insulin were tested by drawing blood from left ventricular.Liver wet weights were measured by freeing liver,and calculated liver index.Rat liver was fixed with 10% formalin and embedded with paraffin.Assessment of hepatic steatosis and inflammation in all rats is measured under light microscope by HE stain.Results Liver tissue pathology showed that the hepatic cells of model group of HFJ and Wistar rats presented diffused macrovesicular steatosis and lobular inflammatory cell infiltration,and HFJ rats were more serious than Wistar rats.Rats in control group showed no abnormal.All rats in model group had elevated levels of AST,ALT,liver index and HOMA-IR,and there was no significant difference between HFJ rats and Wistar rats.Body weigh of the model group of HFJ rats were higher than those in control group(P〈0.01),and there were no significant differences between model group and control group of Wistar rats(P〉0.05).Serum TG and TC of the HFJ rats were obviously higher than that of Wistar rats.Conclusion A fatty liver and insulin resistance model was successfully developed in HFJ inbred rats and Wistar rats by high-fat diet method.Compared with Wistar rats,HFJ rats had spontaneously high blood lipid characteristics,which provided a useful experimental tool for elucidating pathogenesis and treatment of fatty liver.
机构地区 河北医科大学
出处 《四川动物》 CSCD 北大核心 2012年第1期143-146,F0004,共5页 Sichuan Journal of Zoology
基金 河北省科技支撑计划重点项目(09276426D)
关键词 近交系HFJ大鼠 封闭群Wistar大鼠 胰岛素抵抗 脂肪肝 动物模型 HFJ inbred rat Wistar closed colony rat insulin resistance fatty liver animal model
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  • 1张荣,周吕,张宝珍.迷走背核复合区微量注射五肽胃泌素对大鼠胃运动的加强作用[J].生理学报,1993,45(3):279-285. 被引量:3
  • 2汪歌,畅波,李彦,张晓娟,邹岗燕,李京岳,蒋虹,王艳荣.23个实验小鼠和大鼠近交系的保种研究[J].中国实验动物学报,1993,1(2):101-108. 被引量:1
  • 3许如泉,宋运邦.DBA/2小鼠生长和繁殖的研究[J].中国实验动物学报,1997,5(1):57-60. 被引量:3
  • 4李光伟,Step.,L.检测人群胰岛素敏感性的一项新指数[J].中华内科杂志,1993,32(10):656-660. 被引量:2125
  • 5实验动物近交系小鼠、大鼠生化标记检测方法[S].中华人民共和国国家标准GB/T14927.1-2001.
  • 6实验动物近交系小鼠、大鼠皮肤移植法[S].中华人民共和国国家标准GB/T14927.2-2001.
  • 7Benavides F, Glasscock E, Coghlan LG, et al. PCR-based mierosatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains[J]. Lab Amimals, 2001, 35 (2) : 157 - 162.
  • 8Samuel VT, Liu ZX, Qu X, et al. Mechanism of hepatic insulin resistance in non-alcoholic fatty liver disease. J Biol Chem, 2004,279: 32345-32353.
  • 9Neuschwander-Tetri BA, Caldwell SH. Nonalcoholic steatohepatitis:summary of an AASLD Single Topic Conference. Hepatology, 2003,37: 1202-1219.
  • 10Lieber CS, Leo MA, Mak KM, et al. Model of nonalcoholic steatohepatitis. Am J Clin Nutr, 2004, 79: 502-509.

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