摘要
目的:对老鸦瓣进行系统的传粉生物学研究,了解其传粉生殖特性,为今后实现规范化栽培提供依据。方法:于老鸦瓣盛花期观察花部性状;采用不同花粉活力检测方法动态检测花粉活力,用联苯胺-过氧化氢法动态测定柱头授性;估算老鸦瓣花粉-胚珠比(P/O),计算杂交指数值(OCI),依据Dafni及Cruden标准判断老鸦瓣繁育系统类型;进行野外繁育系统验证实验及访花昆虫观察。结果:老鸦瓣花具有典型的百合科花部特征;花药未炸裂前已有一定比例的具活性花粉,在刚开放的1～3 d活力最高,但直至花凋谢时,仍有一部分花粉具有活力,柱头在花开放当天授性最强,随后的5 d内逐渐减弱直至消失;老鸦瓣繁育系统类型为兼性异交,隧蜂属昆虫为老鸦瓣主要传粉昆虫。结论:老鸦瓣属兼性异交类型,异交为主,自交为辅,昆虫为老鸦瓣传粉媒介,风力对老鸦瓣传粉是否起作用需进一步验证。
Objective： Current study on the pollination biology of Tulipa edulis was conducted to investigate its pollination characteristics and to provide references for artificial domestication and breeding of T. edulis. Method： Flowering dynamics, pollina- tors, morphology and structure of flower were observed. Different methods were adopted to evaluate the pollen vitality, and benzidine- H2 02 method was used for estimation of the stigma receptivity. Breeding system was evaluated based on out-crossing index （ OCI）, pol- len-ovule ratio （P/O） and the results of emasculation, hagging and artificial pollination studies. Result： The flower of T. edulis showed typical characteristics of Liliaceae. The pollen remained viable to some extent during all the anthesis and peaked within three days after blossoming. Stigma acceptability peaked in the first day of blossom and dwindled away in the next four days. The type of breeding system of T. edulis was facultative xenogamy, three species of Halictus are the main pollination insects. Conclusion： The type of breeding system of T. edulis was facultative xenogamy, withal cross-polfination give priority to self-pollination, and the insects play a main role on the pollination of 7： edulis, further validation are needed to judge if the wind is helpful to pollination.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第3期293-297,共5页
China Journal of Chinese Materia Medica
关键词
老鸦瓣
光慈姑
传粉生物学
繁育系统
兼性异交
Tulipa edulis
bulb of edible tulip
pollination biology
breeding system
facultative xenogamy
