摘要
目的:测定大黄不同炮制品的致泻效价,对比研究大黄炮制前后的致泻效价差异。方法:以番泻苷B作为对照物质采用小鼠致泻模型对大黄参照物质的致泻效价值进行标化。采用标定的大黄道地参照物作为对照,按照"量反应平行线法(2,2',2″法)"对大黄不同炮制品进行了致泻效价的测定。结果:生大黄的致泻效价为825.22 U.g-1,酒大黄为699.05 U.g-1,熟大黄为459.76 U.g-1,大黄炭的致泻效价无法测得。结论:生大黄经炮制后致泻效价明显降低,酒大黄致泻活性弱于生大黄,熟大黄次之,大黄炭基本丧失泻下活性。
Objective ： To determine the difference on purgative biopotency of different processed products of rhubarb and compare rhubarb before and after preparation. Method ： The prime biopotency of rhubarb reference substance was determined by comparing with the control substance of sennoside B using rat purgative model. Comparing with calibrated rhubarb reference substance, the bioactivity different processed products of rhubarb was determined by the 2,2＇,2 method. Result： The purgative biopotency of crude rhu- barb was 825.22U·g-1 The purgative biopotency of alcohol-processed rhubarb was 699.05 U·g-1 , The purgative biopotency of steamed rhubarb were 459. 76U·g-1. Carbonized rhubarb cannot be determinate. Conclusion： Crude rhubarb showed a significantly declineing purgative biopotency after being processed, alcohol-processed rhubarb showed less than crude rhubarb and followed by steamed rhubarb. Carbonized rhubarb lost almost all purgative bioactivity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第3期302-304,共3页
China Journal of Chinese Materia Medica
基金
科技部"十一五"科技支撑计划项目(2006BAI08B03)
国家自然科学基金杰出青年基金项目(30625042)
关键词
大黄
炮制
致泻效价
rhubarb
processing
purgative biopotency
