摘要
目的探讨丙型肝炎肝外组织丙型肝炎病毒（ｈｅｐａｔｉｔｉｓ Ｃ ｖｉｒｕｓ，ＨＣＶ）感染和复制状态及其意义。方法分别采用免疫组织化学和逆转录多聚酶链反应（ｒｅｖｅｒｓｅ ｔｒａｎｓｃｒｉｐｔａｓｅ ｐｏｌｙｍｅｒａｓｅ ｃｈａｉｎ ｒｅａｃｔｉｏｎ， ＲＴ－ＰＣＲ）方法检测９例丙型肝炎肾、心、胰腺和肠等肝外组织ＨＣＶ多种抗原和正链、负链ＨＣＶ ＲＮＡ。结果 ９例中６例（６６．７％）在肝外组织检出ＨＣＶ多种抗原。抗原定位于细胞浆内。组织内抗原表达阳性细胞数量较少，不同组织间阳性细胞种类、数量和分布略有差异。 ９例患者肝外组织经ＲＴ－ＰＣＲ法均检出ＨＣＶ ＲＮＡ； ３１份标本中２０份（６４５％）检出正链，１１份（３５５％）检出负链。组织中正链和负链ＨＣＶ ＲＮＡ检出与其细胞内病毒抗原表达阳性信号呈较好一致性（Ｋａｐｐａ＞０．４０）。结论ＨＣＶ可能感染肝外组织，但程度轻；还可能在其感染细胞内低水平复制。这种状况可能是肝外组织ＨＣＶ检出率低和检测结果不一致的主要原因之一。
Objective To explore the state of hepatitis C virus(HCV) infection and replication in extrahepatic tissues and its significance in hepatitis C patients. Methods Immunohistochemical assay and reverse transcriptase polymerase chain reaction(RT-PCR) were used respectively to detect HCV antigens(NS3, NSS and CP10) and HCV RNA positive-and negative-strand in kidney, heart, pancreas, and intestine from 9 hepatitis C patients. Results Positive staining for HCV NS3, NSS and CP10 in extrahepatic tissues was found in 6 of the 9(66.7%) patients. The expression of HCV antigens was observed only in plasma of cells. The amount of positive-staining cells was less and there were a few differences between the kind, amounts and distribution of HCV antigen-positive-staining cells in different extrahepatic tissues. HCV RNA was detected in extrahepatic tissues from all the patients by RT-PCR. The detection rates of HCV RNA positive-and negativestrand were 64.5% and 35.5% in 31 extrahepatic tissues specimens, respectively. The positive of HCV RNA positive-and negativestrand detection was coincident with that of HCV antigens detection in these tissues(Kappa > 0.40). Condusions These results suggest a possible presence of HCV infection and replication in extrahepatic tissues. Low--level of HCV extrahepatic infection and replication is possibly a major cause of a low positive rate and inconsistent results of HCV detection in extrahepatic tissues.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第1期40-42,共3页
Chinese Journal of Hepatology
基金
全军医药卫生科研基金!No.98D066)
关键词
丙型肝炎病毒
抗原
聚合酶链反应
肝外组织
Hepatitis C virus
Immunohistochemistry
Polymerase chain reaction
Expression of antigen
