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苯酚-硫酸法测定维吾尔药核桃分心木多糖的含量 被引量:19

Study on Polysaccharides from Uygur Medicine Diaphragma Juglandis Fructus by Phenol-sulfuric Acid Method
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摘要 目的:建立维吾尔药核桃分心木多糖的含量测定方法。方法:以精制后的核桃分心木多糖为研究对象,通过单因素实验,对苯酚-硫酸法测定核桃分心木精制多糖含量的显色条件进行优选,并对该方法进行方法学考察;在最佳显色条件和测定方法下测定分心木中多糖的含量。结果:在5.0~17.5μg/mL范围内,葡萄糖质量浓度与吸光度具有良好的线性关系。其平均回收率为106.55%,相对标准偏差(RSD)值为5.18%。用苯酚-硫酸法在最佳显色条件下测得的核桃分心木多糖含量为5.4%。结论:该测定方法简便灵敏、准确性高、稳定性好、测定结果可靠,可用于核桃分心木的质量控制。 Objective: To study Polysaccharides from Uygur Medicine diaphragma juglandis fructus by phenol sulfuric acid. Methods:l. To select the refined polysaccharides of diaphragrna juglandis fructus as a research object, optimizing the chromo- merit condition of phenol-sulfuric acid method by performing single factor experiment. 2. With the optimized phenol-sulfuric acid methods, the content of polysaceharides from diaphragma juglandis fructus was assayed. Results:The linear relationship of poly- saccharides' concentration was at the range of 5.0~17.5μg/mL. And the average recover was106. 55%, RSD was 5. 18%. The contents of polysaccharides in diaphragma juglandis fructus was 5.4%, assaying with optimized phenol-sulfuric acid method. Conclusion:The assay method which acquired reliable results was proved to be simple, convenient, accurate and stable; and can be used for the quality control of Uygur Medicine diaphragma juglandis fructus.
出处 《亚太传统医药》 2012年第2期35-37,共3页 Asia-Pacific Traditional Medicine
关键词 核桃分心木 苯酚-硫酸法 多糖 含量测定 Polysaccharide Phenol-sulfuric Acid Method Diaphragma Juglandis Fructus
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