摘要
利用液相色谱串联质谱(LC-ESI-MS/MS)测定饲料中盐酸克伦特罗、莱克多巴胺、沙丁胺醇、苯乙醇胺A。通过一系列实验对样品前处理条件进行了优化,样品经乙腈提取、乙酸酸化、净化后,采用HPLC-ESI-MS/MS检测分析,在多反应监测模式(MRM)下,外标法定量。方法的检出限为0.05 mg/kg,各组分浓度在0.001～0.2 mg/kg范围内与定量离子峰面积呈良好的线性关系(r>0.99)。平均加标回收率为61%～75%,测定结果的相对标准偏差为2.78%～4.37%(n=6)。该方法简便、准确,各项技术指标均满足国内外有关饲料中瘦肉精检测要求,可用于饲料中盐酸克伦特罗、莱克多巴胺、沙丁胺醇和苯乙醇胺A残留的分析测定。
A new method was developed for the determination of clenbuterol, ractopamine, salbuterol and phenylethanolamine A by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry. Sample preparation and HPLC-MS/MS analytical conditions were examined and optimized critically by use of a series of experiments study. Samples were extracted by acetonitrile, then cleaned up, and analyzed qualitatively and quantitatively by LC-ESI-MS / MS. The method detection limit （ LOD ） was 0.05 mg / kg. Element concentration was linear with peak area in the range of 0.001-0.2 mg/kg （ r 〉 0.99 ）. The average recoveries ranged from 61% to 75%. The method is simple, efficient, accurate and can meet the requirements of determination for clenbuterol, ractopamine, salbuterol and phenylethanolamine A in feeds.
出处
《化学分析计量》
CAS
2012年第1期37-39,共3页
Chemical Analysis And Meterage
关键词
盐酸克伦特罗
莱克多巴胺
沙丁胺醇
苯乙醇胺A
高效液相色谱-串联质谱
饲料
clenbuterol
ractopamine
salbuterol
phenylethanolamine A
liquid chromatography-electrospray ionization tandem mass spectrometry
feed
