摘要
目的:探讨自噬抑制剂3-甲基腺嘌呤(3-MA)对顺铂(DDP)诱导的卵巢癌SKOV3细胞凋亡的影响。方法:体外培养卵巢癌SKOV3细胞,用PI3K/Akt通路抑制剂3-MA预处理SKOV3细胞3h,再用DDP作用48h,经Annexin V-FITC/PI双染色流式细胞仪检测凋亡率,TUNEL法检测凋亡指数(AI),Western blot检测凋亡蛋白caspase3,caspase9的表达。结果:3-MA预处理后,DDP诱导的卵巢癌SKOV3细胞凋亡率为(37.04±7.15)%,凋亡指数为58.0±5.20,而单用DDP组凋亡率为(10.46±0.65)%,凋亡指数为26.0±3.46。3-MA预处理后,DDP诱导的SKOV3细胞凋亡率和凋亡指数均较单用DDP组明显提高(P<0.05)。caspase3,caspase9蛋白的表达也明显增强。结论:3-MA可上调caspase3,caspase9蛋白的表达,通过内源性途经增强DDP促卵巢癌细胞SKOV3的凋亡活性。
Objective：To study the effect of 3-MA,PI3K/Akt signal inhibitor on apoptosis of ovarian cancer cell induced by DDP.Methods：Cultured ovarian cancer cell line SKOV3 in vitro,pretreated cells with 3-MA for 3 hours,then cultured them with DDP for 48 hours.Apoptosis rate was measured by FCM（flow cytometry） staining with Annexin V-FITC/PI,apoptosis index（AI） was measured by TUNEL,and expression of apoptosis pro-caspase3,caspase9 was detected by Western blot.Results：After pretreated with 3-MA,the apoptosis rate and apoptosis index of SKOV3 treated by DDP were（37.04±7.15）% and（58.0±5.20） respectively,which increased dramatically compared with cells treated only by DDP（P0.05）.Expression of pro-caspase3,caspase9 was enhanced after pretreating with 3-MA in SKOV3 cells.Conclusion：3-MA can up-regulate expression of pro-caspase3,caspase9,and promote apoptosis activity of ovarian cancer cell line SKOV3 induced by DDP through intrinsic apoptotic pathway.
出处
《现代妇产科进展》
CSCD
2012年第1期1-3,共3页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金资助项目(No:30860304)
