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小鼠嗅鞘细胞培养的初步研究 被引量：2

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摘要 目的:研究小鼠嗅鞘细胞(OECs)体外培养的不同方法,为获取数量多、活性好、形态优的嗅鞘细胞提供一定的实验基础。方法:4只出生2～3个月的BALB/c小鼠,经枕骨大孔取嗅球,剥离嗅球外周两层制成细胞悬液。采取未纯化培养对照组、一次差速贴壁结合阿糖胞苷(Ara-c)纯化与多聚左旋赖氨酸包被培养板培养组(纯化包被组)和一次差速贴壁结合Ara-c纯化与未用多聚左旋赖氨酸包被培养板培养组(纯化未包被组)分别培养小鼠OECs,观察其原代和传代培养的细胞生长状况及形态,并进行免疫细胞化学鉴定。结果:纯化未包被组OECs早中期无论数量还是形态都优于纯化包被组,而未纯化对照组的OECs早中期数量明显优于纯化组。结论:一次差速贴壁结合Ara-c纯化未包被进行小鼠OECs培养是一种简捷有效地方法。
作者 花保安 李朝顶 李朝品
机构地区 安徽理工大学医学院病原生物学与免疫学教研室 皖南医学院弋矶山医院外科
出处 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2012年第2期206-208,共3页 Chinese Journal of Cellular and Molecular Immunology
基金 安徽省科技计划年度重点项目(08020303078)
关键词 嗅鞘细胞 细胞培养 形态学 免疫细胞化学
分类号 R392.12 [医药卫生—免疫学]
  • 相关文献

参考文献10

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共引文献19

同被引文献36

  • 1周思朗,屈艳妮,张健,汪森明,张积仁.一种新的细胞计数方法——磺基罗丹明B染色法[J].细胞与分子免疫学杂志,2005,21(5):663-664. 被引量：8
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  • 3张志强,沈铁城,黄永辉,黄秋生,杨勇,龚爱华,张志坚.人血清及神经生长因子对成人鼻黏膜嗅鞘细胞体外增殖的影响[J].江苏大学学报（医学版）,2007,17(1):28-30. 被引量：7
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细胞与分子免疫学杂志
2012年 第2期

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