摘要
以小叶丁香的茎段和叶片为外植体进行植物组织培养。分别以0.1%的升汞和65%的次氯酸钠对外植体进行灭菌,将灭菌的小叶丁香叶片接于不同的培养基中进行脱分化培养;将灭菌的小叶丁香茎段接于不同培养基中,观察腋芽的促生情况;将促生出的小叶丁香的腋芽进行生根培养。结果表明:在0.1%的升汞中,小叶丁香的茎段和叶片最佳灭菌时间均为30s;在65%的次氯酸钠中小叶丁香的茎段和叶片最佳灭菌时间分别为2min和1min;最佳脱分化培养基为:MS+NAA2mg/L+6-BA2mg/L;最佳腋芽促生的培养基为:MS+6-BA2mg/L;最佳生根最适培养基为:MS+NAA0.5mg/L。
Took the stem and leaf of Sytinga microphyla as explants for tissue culture.The explants were sterilized with 0.1% Mercuric chloride and 65% sodium hypochlorite respectively.Then the sterilized explants were subjected to different culture media for dedifferentiation to observe different effects on promoting axillary bud growth.Then root growth cultivation was carried out on the axillary buds.The results showed as below：in 0.1% Mercuric chloride the optimum time for sterilization of stem and leaf were both 30s;But in 65% sodium hypochlorite the best time for sterilization were 2min and 1min separately.The best media for dedifferentiation is MS＋NAA2mg/L＋6-BA2mg/L.The best media to promote the growth of axillary bud is MS＋6-BA2mg/L.The best media for root growth is MS＋NAA0.5mg/L.
出处
《中国林副特产》
2012年第1期32-34,共3页
Forest By-product and Speciality in China
关键词
小叶丁香
组织培养
Sytinga microphyla
Tissue culture
