摘要
实验通过研究卵母细胞的核相及皮层颗粒的分布，探讨按照卵泡大小（直径小于１ ｍ ｍ 、１～３ ｍ ｍ 和大于３ ｍ ｍ ）和卵丘细胞层多少分类后的不同绵羊卵母细胞在体外培养过程中的核质成熟情况．结果表明：１）小卵泡卵经体外培养２４ ｈ 后的核质成熟率分别为４５．９％ 和２８．２％ ，远低于大（９３．５％ ，６６．７％ ）、中（８５．１％ ，５１．０％ ）两类卵泡卵．２）大卵泡卵在体外培养２０ ｈ 已完成核质成熟，较中等卵泡卵提前４ ｈ．３）带有３ 层以上致密卵丘细胞及１～３ 层较疏松卵丘细胞的卵母细胞可以经过体外培养完成核质成熟，而裸卵及卵丘细胞已扩散的卵母细胞是退化的卵母细胞，其中裸卵的退化程度更高．
This study was designed to examine the nuclear and cytoplasmic maturation of sheep oocytes classified according to follicular sizes and the layers of cumulus cells. Results indicated that:1)the capability of maturation of small follicular oocytes is lower than that of big and medium follicular oocytes significantly. 2)big follicular oocytes matured 4 hours earlier than medium follicular oocytes.3)in four categories of oocytes classified according to the layers of cumulus cells: 1) and 2) categories of oocytes can mature after in vitro culture but 3) and 4) categories of oocytes degenerated.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
2000年第1期101-106,共6页
Journal of Inner Mongolia University：Natural Science Edition
基金
八五攻关项目基金
