摘要
目的探讨当归拈痛汤加味膏外敷在佐剂性关节炎(AA)大鼠关节中对低氧诱导因子-1α(HIF-1α)的表达及滑膜凋亡的影响。方法 40只wistar大鼠随机分为正常组、模型组、治疗组及对照组。建立AA模型,给药后18d,取踝关节行HE染色及HIF-1α免疫组化染色,流式细胞仪检测滑膜细胞的凋亡变化。分析HIF-1α、滑膜凋亡在AA中的相关性及与节炎活动指标、滑膜病理之间的关系。结果给药18d后,治疗组HE染色显示明显减轻滑膜增殖以及炎症反应,免疫组化显示治疗组HIF-1α明显表达降低,流式细胞仪检定显示治疗组滑膜细胞凋亡率明显升高。结论HIF-1α的表达与滑膜细胞凋亡率呈明显负相关,该膏剂通过抑制HIF-1α活性,从而调控一系列下游靶基因,如滑膜细胞凋亡等,此可能是其抑制滑膜增生的作用机制之一。
Objective： To explore the affect of Modified Dangguiniantong Paster on HIF-1α and synovium apoptosis of adjuvant arthritis（AA） rats.Methods： 40 wistar rats were divided randomly into the normal group,the model group,the treatment group and the control group.After therapies for 18 days,the ankle joint and the HIF-1α were stained and detected by the flow cytometry.The relativity between the HIF-1α and the synovium apoptosis and the relationship among above indices,the arthritis indices and the synovium pathological change were observed.Results： After drug administration to rats for 18 days,there were remarkable reductions of the synovium cell proliferation and inflammatory reactions.The HIF-1α in the treatment group was decreased obviously from the immunohistochemistry.But the ratio of synovium cell apoptosis in the treatment group was increased significantly by the flow cytometry.Conclusion： There is negative correlation in the HIF-1α expression and the ratio of synovium apoptosis.The mechanism of Modified Dangguiniantong Paster on reduction synovium cell proliferation could be through decreasing the activity of HIF-1α and regulation of downstream target genes.
出处
《中国中医急症》
2012年第2期226-228,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
新疆医科大学研究生创新基金(MC2010-33)
关键词
类风湿关节炎
佐剂性关节炎
低氧诱导因子-1Α
滑膜
细胞凋亡
当归拈痛汤
Reumatoid arthritis
Adjuvant arthritis
Hypoxia inducible factor-1α
Synovial membrane
Cell apoptosis
Dangguiniantong Decoction
