摘要
采用未有百菌清用药史的土壤定向培养49d,分离出50株百菌清耐受菌株,经初筛、复筛后得到1株降解能力最强的细菌BJQ2,经形态学、生理生化试验、16SrDNA序列同源性比对及系统发育树的分析,初步确定为阴沟肠杆菌（EnterobactercloacaeB9）,BJQ2可以以百菌清为唯一碳源于基础培养基中生长。BJQ2降解百菌清的最适宜条件为：培养温度30~35℃,初始pH值为7.0~8.0,百菌清的初始浓度为20mg·L-1,选用菌体母液（OD600=0.35）,以10%的接种量接种,6d后提取百菌清,利用气相色谱进行检测分析,计算后发现百菌清的降解率可以达到79.2%。
Directionally cultivated for 49 days in the soil with no history of chlorothalonil （TPN, 2,4,5,6-tetrachloro-l,3-isophthalonitrile ） application, 50 chlorothalonil-tolerant bacteria strains were isolated. The highest concentration of ehlorothalonil was up to 600 mg·L^-1. BJQ2, one with the best degradability among those strains, was obtained after prescreening and affination. It was initially identified as Enterobacter cloacae B9 on the basis of morphological approach, physiological-biochemical experiment, homology comparison of 16S rDNA and the analysis of phylogenetic tree. BJQ2 could be bred in the basic medium by using chlorothalonil as the unique carbon and energy source. The most appropriate condition for BJQ2 to degrade chlorothalonil was as follows ： Breeding temperature was 30-35℃, original pH value was 7.0-8.0, original TPN concentration was 20 mg. L-1 and the inoculation amount was 10 percent（primary mother liquor, OD600=0.35 ） in the experiment （ V/V ）. In this condition, chlorothalonil was extracted after 6 days. And its degradation rate was calculated to arrive at 79.2 percent by check-ing and analyzing with gas chromatography.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2012年第2期306-311,共6页
Journal of Agro-Environment Science
基金
2011第七届北京市教学名师项目
2010年度北京市属高等教育学校人才强教深化计划"学术创新人才(PHR201006124)"
北京市属高等学校人才强教深化计划资助项目"蔬菜优质安全生产理论与技术体系创新研究(PHR200907136)"
北京果园常用农药残留检测技术研究与应用(Z09060500600906)
农产品加工及贮藏工程北京市重点建设学科(PXM2009-014207-078172)
首都农产品安全产业技术研究院成果转化项目(PXM2012-014207-000019)
北京市科技计划项目"农产品中有害微生物及农残检测与控制关键技术研究及应用"
关键词
百菌清
阴沟肠杆菌
生物降解
影响因素
chlorothalonil
Enterobacter cloacae B9
biodegradation
influencing factors
