摘要
目的观察酸敏感离子通道(ASICs)在过敏性紫癜(HSP)患儿血管内皮细胞中的表达并探讨其在血管炎性损伤中的作用及可能的调节机制。方法①采用免疫组化法观察HSP患儿及正常儿童皮肤血管内皮细胞胞浆ASIC1a、ASIC2a、ASIC3的表达情况;②培养人脐静脉内皮细胞(HU-VECs),分为空白对照组(不含血清的1640培养)、正常血清组、HSP血清组、甲泼尼龙干预组,采用半定量RT-PCR法检测HUVECs ASIC1a、ASIC2a、ASIC3 mRNA表达,Western blot检测其蛋白表达及甲泼尼龙干预对ASICs表达的影响。结果①免疫组化显示HSP患儿皮肤血管内皮细胞胞浆ASIC1a、ASIC3表达较正常对照组显著增多(P<0.05),ASIC2a少量表达与正常对照组差异无统计学意义;②HSP血清组ASIC1a、ASIC3 mRNA及蛋白表达较空白对照组、正常血清组明显增高(P<0.01),而甲泼尼龙干预组明显低于HSP血清组(P<0.01),且与正常血清组比较差异无统计学意义;③ASIC1a、ASIC3蛋白表达正常血清组较空白对照组增高(P<0.05),但正常血清组和空白对照组比较,mRNA表达差异无显著性;④ASIC2a mRNA及蛋白表达各组差异均无统计学意义。结论 HSP患儿血清刺激可使HUVECsASIC1a及ASIC3表达增高,ASICs可能参与了过敏性紫癜皮肤血管的损伤过程。甲泼尼龙可能通过抑制体内ASICs的高表达而缓解血管损伤。
Objective To observe the expression of acid-sensingion channels（ASICs） in venous endothelial cells of Henoch-Schnlein purpura（HSP） patients and discuss the action and mechanism of inflammatory endothelial damage mediated by ASICs.Methods Immunohistochemistry methods were used to investigate the expression of ASIC1a,ASIC2a,ASIC3.Human umbilical venous endothelial cells（HUVECs） were cultured in 4 different conditional media without fetal calf serum,simply cultured group,normal serum group,HSP serum group,and methylprednisolone treatment group.The transcriptional levels of ASICs genes were semi-quantitatively assayed by RT-PCR,and ASICs proteins of methylprednisolone treated were determined by Western blot.Results The expression of ASIC1a,ASIC3 in venous of skin of HSP patients were significantly increased（P0.05）.The levels of ASIC1a,ASIC3 mRNA and proteins were significantly increased in the HSP group（P0.01）,but not in ASIC2a.Compared with HSP serum group,the expression levels of ASIC1a,ASIC3 in methylprednisolone treatment group were significantly decreased（P0.01）.Conclusion This study suggests a excitive role of ASICs in the damage of endothelial cells in HSP.Some factors may be involved in serum of children with HSP that can activate HUVECs to produce ASIC1a and ASIC3.Methylprednisolone can protect vascular damage by inhibitory regulation of ASICs high expression.
出处
《安徽医科大学学报》
CAS
北大核心
2012年第2期189-193,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:11040606M168)
