摘要
目的探讨激活素受体相互作用蛋白(Activin receptor-interacting protein,ARIP)1及2(ARIP1,2)在小鼠巨噬细胞RAW264.7中的表达及作用。方法采用免疫细胞化学染色法检测RAW264.7细胞中ARIP1,2的表达;将质粒CAGA-lux、CMV-gal分别与表达质粒pcDNA3-ARIP1、pcDNA3-ARIP2或空质粒pcDNA3共转染RAW264.7细胞,应用激活素A刺激,检测细胞内报告基因转录荧光素酶的活性;实时定量RT-PCR检测ActRⅡA mRNA的表达。结果免疫细胞化学染色结果显示,小鼠巨噬细胞系RAW264.7细胞表达ARIP1,2;RAW264.7细胞过表达ARIP1,2均能抑制Activin A诱导的特异基因转录;过表达ARIP2可抑制RAW264.7细胞ActRⅡA mRNA的表达,而过表达ARIP1对ActRⅡA mRNA的表达无明显影响。结论 ARIP1,2在小鼠巨噬细胞中共表达,并具有下调激活素信号传导的作用,但其作用方式不同。
Objective To investigate the expression and role of activin receptor-interacting proteins 1, 2 （ARIP1, 2） in mouse macrophages. Methods The expression of ARIP1, 2 was determined by immunoeytochemical staining. RAW264. 7 cells were eo-transfected with plasmids CAGA-lnx and CMV-gal, plus plasmids pcDNA3-ARIP1, pcDNA3-ARIP2 or empty plasmid pcDNA3, respectively, then stimulated with activin A, and determined for transcription activity of report gene. The expression of ActR Ⅱ A mRNA was determined by RT-PCR. Results Immunocytochemical staining proved that ARIP1,2 were expressed in RAW264. 7 cells. The overexpressions of ARIP1, 2 inhibited the transcription of specific gene induced by activin A. The overexpression of ARIP2 inhibited, while that of ARIP1 showed no significant effect on the expression of ActR Ⅱ A mRNA in RAW264. 7 ceils. Conclusion ARIP1, 2 were coexpressed in mouse maerophages, which down-regulated the signal transduction of activin by different action modes.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第2期161-163,167,共4页
Chinese Journal of Biologicals
基金
国家自然科学基金(30801005
30901323
30901329)
吉林省科技厅课题(20080160
20090742)
