摘要
目的观察中药筋脉通胶囊对糖尿病大鼠坐骨神经形态测量学及核转录因子κB(nuclear factor kappa B,NF-κB)蛋白表达的影响。方法采用链脲佐菌素糖尿病大鼠模型,随机分为模型组和筋脉通组及神经妥乐平组,设立正常对照组。灌胃16周后取坐骨神经进行形态测量学分析,免疫组化法测定其NF-κB蛋白表达。结果与正常对照组相比,模型组大鼠的有髓神经纤维密度、小纤维密度减少,小纤维比率下降,有髓神经纤维平均横截面积、轴索平均横截面积增大,差异显著(P<0.01);筋脉通组、神经妥乐平组的有髓神经纤维密度、小纤维密度、小纤维比率均较模型组显著改善(P<0.05);筋脉通组的有髓神经纤维平均横截面积、轴索平均横截面积较模型组改善显著(P<0.05)。模型组大鼠NF-κB蛋白的表达较正常组显著升高(P<0.01);筋脉通组、神经妥乐平组上值均较模型组降低(P<0.05)。结论筋脉通胶囊可改善糖尿病大鼠坐骨神经的形态测量学异常,减少NF-κB的异常高表达。
Objective To study the effects of Jinmaitong Capsule（JMT） on morphology and expression of nuclear factor kappa B（NF-κB） of sciatic nerves in streptozotocin-induced diabetic rats.Methods Streptozotocin-induced diabetic rats were randomly divided into 3 groups including model group（DM group）,JMT group and Neurotropin group（Ntp group）.Normal rats were nsed as normal control group.All rats were given intragastric administration for 16 weeks.The morphometric studies of sciatic nerves were investigated.I immunohistochemical method was applied to detect the NF-κB＇s expression in sciatic nerves.Results Compared with the normal control group,the density of myelinated fibers and small myelinated fibers,the rate of small myelinated fibers,the size of myelinated fibers and axon cross sections in the DM group were significantly reduced（P0.01）.The indexes above in 2 treatment groups were significantly improved compared with the DM group.The size of axon cross sections in the JMT group were more significantly improved than in the DM group（P0.05）.The NF-κB＇s expression in the DM group was significantly higher than in the normal control group（P0.01）,while which in treatment groups were lower than in the DM group（P0.05）.Conclusion JMT could improve the pathological morphology of sciatic nerves and down-regulate the expression of NF-κB in DPN rats.
出处
《环球中医药》
CAS
2012年第2期100-104,共5页
Global Traditional Chinese Medicine
基金
北京市自然科学基金项目(7082077)
关键词
糖尿病周围神经病变
核转录因子ΚB
形态测量学
筋脉通
Diabetic peripheral neuropathy
Nuclear factor kappa B
Morphology
Traditional Chinese medicine Jinmaitong Capsule
