摘要
目的对火焰原子吸收光谱法测定铁、锌、钙元素进行方法学评价,了解本地区儿童体内铁、锌、钙含量元素水平。方法绘制标准曲线,进行精密度和回收率评价;采集儿童末梢血,用含1%HNO3的0.5%曲拉通X-100(Tri-onX-100)1∶200稀释检测铁、锌、钙元素含量。结果采用该方法批内变异系数铁元素3.7%,锌元素1.9%,钙元素1.5%,批间变异系数铁元素4.5%,锌元素2.5%,钙元素2.7%,回收率为铁元素96.4%,锌元素98.0%,钙元素96.6%。儿童缺铁的百分率为13%,缺锌的百分率为10%,缺钙的百分率为10%。结论火焰原子吸收光谱法测定微量元素重复性好,回收率符合要求,可直接用于全血元素含量的测定,结果满意可靠。
Objective To evaluate the methodology of flame atomic absorption spectrometry used to determine Iron,Zinc and Calcium and to understand the Iron,Zinc and Calcium element levels of children in local.Methods The standard curve was drawn,and the precision degree and recovery rate were evaluated;the peripheral blood of children was collected,and the Iron,Zinc and Calcium elements were diluted and detected with 0.5% TrionX-100 containing 1% HNO3,with a ratio of 1∶200.Results With this method,the in-batch variation coefficient was 3.7% for Iron,1.9% for Zinc and 1.5% for Calcium,the between-batch variation coefficient was 4.5% for Iron,2.5% for Zinc and 2.7% for Calcium,and the recovery rate was 96.4% for Iron,98.0% for Zinc and 96.6% for Calcium.The percentage of children with Iron deficiency was 13%,Zinc deficiency was 10% and calcium deficiency was 10%.Conclusion Flame atomic absorption spectrometry shows good reproducibility and satisfactory recovery rate in the determination of trace elements,and it can be directly used for the determination of elements in whole blood,and has satisfactory and reliable results.
出处
《中国医药导报》
CAS
2012年第5期112-113,共2页
China Medical Herald
基金
江苏省徐州市科技局发展基金项目(项目编号:XF10C008)
关键词
火焰原子吸收光谱
全血
儿童
微量元素
Flame atomic absorption spectrometry
Whole blood
Children
Trace elements
