摘要
目的:探讨肿瘤患者化疗前外周血有核细胞中核因子E2相关因子2(nuclear factor erythroid-2-related factor2,Nrf2)mRNA表达水平的个体差异与化疗所致骨髓抑制程度的相关性。方法:采用半定量RT-PCR法检测30例肿瘤患者化疗前外周血有核细胞中Nrf2mRNA的表达水平,并分析其与肿瘤患者化疗后骨髓抑制程度的相关性。结果:肿瘤患者化疗前外周血单个核细胞中Nrf2 mRNA表达水平存在明显的个体差异;其在化疗后发生0～1度白细胞或中性粒细胞减少患者中的表达水平明显高于2～4度的患者(P<0.05),但与患者的年龄、性别、治疗方案及肿瘤类型等无相关性。相关性分析表明,外周血单个核细胞中Nrf2 mRNA的表达水平与白细胞减少(r=-0.448,P=0.013)和中性粒细胞减少(r=0.493,P=0.006)呈显著负相关。结论:化疗前外周血单个核细胞中Nrf2 mRNA表达水平与化疗所致骨髓抑制具有相关性,因此,其可能成为一种预测化疗所致骨髓抑制程度的理想标志物,用于指导临床方案的选择及骨髓抑制的早期防治。
Objective：To investigate the interindividual variation of nuclear factor erythroid 2-related factor 2（Nrf2） mRNA expression level in the peripheral blood nucleated cells and its correlation with the severity of chemotherapy-induced myelosuppression.Methods：The expression level of Nrf2 mRNA in the peripheral blood nucleated cells from 30 cases of cancer was detected by semi-quantitative RT-PCR before chemotherapy,and the association between the Nrf2 mRNA expression and the chemotherapy-induced myelosuppression was analyzed.Results：The Nrf2 mRNA expression level in the peripheral blood mononuclear cells exhibited significant interindividual differences.The Nrf2 mRNA expression level was significantly higher in the patients with grades 0-1 leukopenia or neutropenia than that in the patients with grades 2-4（P0.05）,but it was not associated with the age,gender,chemotherapeutic regimen and the type of tumor.The association analysis showed that the Nrf2 mRNA expression level in the peripheral blood mononuclear cells was negatively associated with leukopenia and neutropenia（r =-0.448,P = 0.013;r =-0.493,P = 0.006）.Conclusion：The expression level of Nrf2 mRNA in the peripheral blood mononuclear cells is significantly correlated with chemotherapy-induced myelosuppression.It may be employed as an ideal marker to predict the severity of chemotherapy-induced myelosuppression and helpful for clinical decision-making and early prevention and treatment of myelosuppression.
出处
《肿瘤》
CAS
CSCD
北大核心
2012年第2期124-129,共6页
Tumor
关键词
肿瘤
抗肿瘤联合化疗方案
白细胞减少
粒细胞减少
核因子E2相关因子2
Neoplasms
Antineoplastic combined chemotherapy protocols
Leukopenia
Neutropenia
Nuclear factor erythroid 2-related factor 2
