摘要
目的:研究IL-4、GM-CSF和香菇多糖对口腔鳞癌浸润淋巴细胞(TIL)的体外培养和细胞毒效应的影响。方法:将手术切取的标本通过酶消化加机械分离法提取TIL,分成4组。以单纯加IL-2培养的TIL为对照组,另3组在加IL-2的基础上分别加入IL-4、GM-CSF和香菇多糖进行培养。每3d计数1次并绘制细胞生长曲线图。于对数生长期用MTT法检测TIL的杀瘤活性并进行统计学比较。结果:添加IL-4和GM-CSF的TIL增殖速度明显大于对照组和香菇多糖组,对数生长期出现早且持续时间较长,细胞增殖量也较对照组和香菇多糖组多。MTT法检测TIL杀瘤活性显示,IL-4和GM-CSF组的杀瘤活性强于对照组和香菇多糖组,差异均显著。结论:GM-CSF、IL-4协同IL-2能够提高TIL的增殖速度,促进TIL的增殖,且能在一定程度上解除TIL的免疫抑制,提高其杀瘤活性。
Objective： To explore the effects of IL-4 , GM-CSF or LNT on the proliferation and cytotoxicity of TILs. Methods： We get TILs by handling surgical specimens with enzymatic digestion or mechanical separation. The contrast group was cultured in IL-2 only; the other three groups were cultured in IL-4, GM-CSF or LNT respectively besides IL-2. Calculate the number of TILs each 3 days and protract the growth curve of it. MTT assay was used to examine TILs cytotoxicity during the logarithmic growth phase. Results： The proliferation velocity of the two groups which were cultured in IL-2 appended IL-4 or GM-CSF was much more higher than that of the other two groups. Logarithmic growth phase of the two groups appeared earlier and lasted longer than the contrast group. As a result of MTT assay, cytotoxicity of those two groups was also higher. Conclusion： IL-4 and GM-CSF can increase the proliferation of TILs when adding to the medium with IL-2. Both of them can also enhance the cytotoxicity of TILs by mitigating the immunosuppression probably.
出处
《口腔医学研究》
CAS
CSCD
2012年第2期173-175,共3页
Journal of Oral Science Research
