摘要
After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.
After co-cultrured osteoblast with fl-TCP ceramics, the cellular proliferating, mineralization and osteocalcin expression were studied. MTT assay showed that fl-TCP ceramics had no affect on cellular proliferating. Laser scanning confocal detection showed that fl-TCP ceramics could increase the mineralization level of osteoblast. Furthermore, RT-PCR showed that fl-TCP could increase the expression level of osteocalcin. Those results indicate β-TCP ceramics had perfect biocompatibility and increased the mineralization of osteoblast to accelerate osteogenesis by means of affecting the expression of genes involving in osteogeneticprocess.
作者
QI Zhitao
ZHANG Qihuan
ZHENG Qiang
DAI Honglian
WANG Zisheng
QIU Ming
LI Shipu
齐志涛;张启焕;ZHENG Qiang;DAI Honglian;WANG Zisheng;QIU Ming;LI Shipu(School of Chemical and Biological Engineering,Yancheng Institute of Technology;The Bio-material Research and Engineering Center,Wuhan University of Technology;Yancheng Tianbang Feed Technology Co.Ltd,Yancheng 224056,China)
基金
the Research Fund of Key Labortary for Advanced Technology in Environmental Protection of Jiangsu Province (No. AE201037)
the Foundation for Talent Recruitment of Yancheng Institute of Technology (No. XKR2011007)
the "973" Chinese National Key Fundamental Research and Development Program (No. G1999064701)
