摘要
目的建立测定生地茶止咳合剂中岩白菜素含量的反相高效液相色谱法。方法采用Phenomenex C18-ODS(250mm×4.6mm,5μm)色谱柱,流动相:甲醇-水(23∶77),流速:1.0mL.min-1,检测波长:275nm,柱温:30℃。结果岩白菜素在0.040 8～0.408μg线性关系良好,(r=0.999 9);平均回收率为98.5%(n=9)。结论该方法操作简便、快速,适用于生地茶止咳合剂中岩白菜素的含量测定。
Objective To establish an RP-HPLC method for the determination of bergenin in Shengdicha Zhike Heji.Methods The column was Phenomenex C18-ODS（250 mm×4.6 mm,5 μm）.The mobile phase was methanol-water（23∶77） with a flow rate of 1.0 mL·min-1.The detection wavelength was 275 nm.The column temperature was 30 ℃.Results The calibration curve was linear over 0.040 8-0.408 μg（r=0.999 9）.The average recovery of bergenin was 98.5%（n=9）.Conclusion The method is simple,quick,and suitable for the content determination of bergenin in Shengdicha Zhike Heji.
出处
《中南药学》
CAS
2012年第2期96-98,共3页
Central South Pharmacy
