摘要
目的:观察尼氟灭酸(NFA)对增殖的大鼠气道平滑肌细胞(3-5代ASMC)原癌基因c-fos表达的影响。方法:分离大鼠气道平滑肌细胞,放入含15%胎牛血清的培养基中培养,第35代细胞用于实验。向细胞中加入不同浓度NFA(0、10、50、100μmol/L),分别作用1、2、3h后,收集细胞,提取总RNA,通过逆转录-聚合酶链反应(RT-PCR)的方法检测原癌基因c-fos的表达。结果:高、中、低浓度的NFA组气道平滑肌细胞中c-fos基因的表达水平在3个时间点均明显低于对照组,且随着NFA浓度的增高,表达量逐渐减少。结论:NFA对增殖的大鼠气道平滑肌细胞c-fos基因的表达有明显的抑制作用。
Objective：To observed the effects of niflumic acid （NFA） on the expression of c-fos in the proliferative airway smooth muscle cells （ASMCs） of experimental rat. Methods：ASMCs of rats were isolated and cultured in DMEM containing 15% fetal bovine serum. Well growth cells after 3-5 passage were in the experiment. The cells were cultured in DMEM medium containing different concentrations of NFA （0,10,50, 100 umol/L ） for 1,2 and 3 hours. Then total RNA of ASMCs was isolated, and the c-fos gene expression levels were detected by reverse transcription polymerase chain reaction. Results：The c-los gene expression levels of ASMCs in high,middle and low concentrations of NFA groups were significantly lower than those in control group. With the increase of NFA concentration, the expression of c-fos decreased. Conclusion： Niflumic acid could significantly inhibit the gene expression of c- fos in ASMCs.
出处
《内科急危重症杂志》
2012年第1期52-54,共3页
Journal of Critical Care In Internal Medicine
