摘要
本试验旨在了解鸡源性沙门氏菌分离株多重耐药与Ⅰ类整合子及耐药基因的携带关系。采用K-B纸片法对29株鸡源性沙门氏菌分离菌株进行10种抗菌药物敏感试验;应用PCR技术对分离菌株进行Ⅰ类整合子及耐药基因检测。29株分离株中有13株对2种以上抗菌药物耐药,属于多重耐药株,氨苄西林-四环素-头孢唑啉-复合磺胺是主要多重耐药谱;13株多重耐药菌中有8株携带Ⅰ类整合子,blatem-1、tetA和tetB基因检出最高。结果表明沙门氏菌多重耐药性与整合子的携带之间关系密切,耐药表型测定结果与耐药基因检测结果基本一致。
To determine the relationship between integron I and drug resistance genes multidrug-resistance Salmonella isolated from chickens,the sensitivity tests of 29 strains to 10 antibacterials were performed by Kirby-Bauer method,the integron I and resistance genes were detected by PCR. There were 13 strains were multidrug resistance strains which showed resistance to more than two kinds of antibacterials. Ampicillin-Tetracycline-Cefazolin Compound Sulfadiazine was the main muhidrug resistance spectrum,and there were 8 multidrug resistance strains carried integron I ,and blatme-l,tetA and tetB were the predominant genes. These results suggested that multidrug-resistance Salmonella had a close correlation with carried integron, the detection of resistance phenotype agreed with the detection of resistance genes.
出处
《中国家禽》
北大核心
2012年第4期29-32,共4页
China Poultry
基金
黑龙江省教育厅自然科学面上项目(11541030)
关键词
鸡
多重耐药
沙门氏菌
Ⅰ类整合子
耐药基因
chicken
multidrug resistance
Salmonella
integron I
resistance genes
