摘要
选择藻华现象严重的巢湖作为采样点,取3个不同位置的水样通过0.22μm的纤维滤膜过滤,培养后加入适应期的藻液中,取黄化藻液作为分离菌种的材料,初筛菌株经反复试验获得有较强抑藻能力的菌株,经生理生化鉴定及16S rDNA分子鉴定其种属。初筛得到45个菌株,有两个菌株WD1和WD2表现出溶藻作用。两株细菌的菌液经离心、高温灭菌、细胞破碎等处理对供试藻鱼腥藻也均有不同程度的抑制作用。分离得到两株溶藻细菌,WD1为约氏不动杆菌,WD2为门多萨假单胞菌。
Water samples were collected at three sampling points of Chaohu lake, a eutrophic and algal bloom lake. The samples were filtrated with 0.22 p.m fiber filter membrane, then the filter membrane was put into liquid LB medium. The culture was mixed with test anabaena algae which was in adaptive phase. Strains were isolated from the yellowing algae culture via repeat tests. Physiology biochemistry experiments and 16S rDNA molecular detection were carried out for strain identification. From 45 isolates, two bacterial strains WD1 and WD2 presented relatively strong ability of algae inhibition. When treated the culture of these two strains with filtration, centrifugation, high-temperature sterilization and cell breakage respectively, algae growth were inhibited in various degrees. Two algicidal bacteria were isolated. WD1 and WD2 were assigned to Acinetobacter johnsonii and Pseudomonas menclocina, respectively.
出处
《生物学杂志》
CAS
CSCD
2012年第1期42-46,共5页
Journal of Biology
基金
安徽省自然科学基金项目(090413083)
安徽省高校优秀青年人才基金项目(2004jq114)
关键词
溶藻细菌
鱼腥藻
约氏不动杆菌
门多萨假单胞菌
巢湖
Algicidal bacteria
Anabaena
Acinetobacter johnsonii
Pseudomonas mendocina
Chaohu Lake
